Analysis of the organization and nucleotide sequence of the chromosomal gene for the beta-subunit of rat thyrotropin.
Analysis of the organization and nucleotide sequence of the chromosomal gene for the beta-subunit of rat thyrotropin.
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大鼠促甲状腺素β亚基染色体基因的组织和核苷酸序列分析。
DOI：
10.1089/dna.1986.5.299
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Maurer,RA
中科院分区：
文献类型：
--
作者：
Croyle,ML;Bhattacharya,A;Gordon,DF;Maurer,RA
从噬菌体λ中克隆的大鼠DNA片段文库中分离出大鼠促甲状腺素β亚基基因。已经确定了该基因的完整核苷酸序列，包括5 ‘和3 ’侧翼区域的一部分。大鼠TSH-β基因含有约4879个核苷酸，最终导致产生约554个核苷酸的mRNA，不包括3 ' poly(a)通道。第一个外显子仅代表mRNA的5 '未翻译序列，与第二个外显子被一个非常大的3.9 kbp的中间序列隔开。第二和第三外显子由一个小的377 bp的中间序列分开。全基因组DNA的Southern blot分析表明，大鼠基因组包含与克隆基因相似的序列，表明克隆过程中没有发生重排。
The gene for the β-subunit of rat thyrotropin has been isolated from a library of rat DNA fragments cloned in bacteriophage λ. The complete nucleotide sequence of the gene has been determined including a portion of 5′- and 3′-flanking regions. The rat TSH-β gene contains approximately 4879 nucleotides which ultimately lead to the production of a mRNA of about 554 nucleotides, exclusive of the 3′ poly(A) tract. The first exon which represents only 5′ untranslated sequences of the mRNA, is separated from the second exon by a very large, 3.9-kbp intervening sequence. The second and third exons are separated by a small, 377-bp intervening sequence. Southern blot analysis of total genomic DNA demonstrated that the rat genome contains sequences similar to the cloned gene, suggesting that no rearrangements occurred during the cloning process.