Plasma lipoproteins as carriers of phylloquinone (vitamin K1) in humans
Plasma lipoproteins as carriers of phylloquinone (vitamin K1) in humans
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DOI：
10.1093/ajcn/67.6.1226
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发表时间：
1998-06-01
影响因子：
7.1
通讯作者：
Schaefer, EJ
中科院分区：
文献类型：
--
作者：
Lamon-Fava, S;Sadowski, JA;Schaefer, EJ
本研究的目的是表征叶绿醌（维生素K-1）的吸收和转运的血浆脂蛋白。26名年龄在20-78岁的健康受试者（11名男性和15名女性）口服叶绿醌，剂量为1.43或50 μ g/kg体重，并在富含脂肪的膳食中含有1.0 g/kg体重的脂肪、碳水化合物和蛋白质以及7.0 mg胆固醇/kg体重。在基线（0 h）和餐后3、6、9和12 h采集血液，用于测量血浆脂质和血浆及脂蛋白亚组分中的叶绿醌浓度。在两组受试者中，血浆和富含三酰甘油的脂蛋白组分（由乳糜微粒和VLDL组成）中的三酰甘油浓度在3 h后达到峰值。血浆叶绿醌浓度在6 h达到峰值。在基线和餐后阶段，> 53%的血浆叶绿醌由富含三酰甘油的脂蛋白部分携带。在11名受试者中的9名补充50 μ g叶绿醌/kg，血浆脂蛋白通过连续超离心分离。在这些受试者中，LDL和HDL携带的血浆叶绿醌分数分别从3小时的3%和4%逐渐增加到12小时的14%和11%。我们的数据表明，而富含三酰甘油的脂蛋白是叶绿醌的主要载体，LDL和HDL可能携带这种维生素的小部分。
The purpose of this study was to characterize the absorption and transport of phylloquinone (vitamin K-1) by plasma lipoproteins. Twenty-six healthy subjects (11 men and 15 women) aged 20-78 y received phylloquinone in the amount of either 1.43 or 50 mu g/kg body wt orally with a fat-rich meal containing 1.0 g/kg body wt of fat, carbohydrate, and protein and 7.0 mg cholesterol/kg body wt. Blood was obtained at baseline (0 h) and 3, 6, 9, and 12 h after the meal for the measurement of plasma lipid and phylloquinone concentrations in plasma and lipoprotein subfractions. In both groups of subjects, triacylglycerol concentrations peaked after 3 h in plasma and in the triacylglycerol-rich lipoprotein fraction, composed of chylomicrons and VLDLs. Plasma phylloquinone concentrations peaked at 6 h. At baseline and during the postprandial phase, > 53% of plasma phylloquinone was carried by the triacylglycerol-rich lipoprotein fraction. In 9 of the 11 subjects supplemented with 50 mu g phylloquinone/kg, plasma lipoproteins were isolated by sequential ultracentrifugation. In these subjects the fraction of plasma phylloquinone carried by LDLs and by HDLs increased progressively from 3% and 4% at 3 h to 14% and 11% at 12 h, respectively. Our data indicate that whereas triacylglycerol-rich lipoproteins are the major carriers of phylloquinone, LDL and HDL may carry small fractions of this vitamin.