Opening of compacted chromatin by early developmental transcription factors HNF3 (FoxA) and GATA-4
Opening of compacted chromatin by early developmental transcription factors HNF3 (FoxA) and GATA-4
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DOI：
10.1016/s1097-2765(02)00459-8
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发表时间：
2002-02-01
期刊：
影响因子：
16
通讯作者：
Zaret, KS
中科院分区：
文献类型：
--
作者：
Cirillo, LA;Lin, FR;Zaret, KS
转录因子HNF3 （FoxA）和GATA-4是已知最早在胚胎肝前体细胞中结合白蛋白基因增强子的转录因子。为了了解它们如何进入沉默染色质中的位点，我们组装了含有白蛋白增强子序列的核小体阵列，并将它们与连接组蛋白紧密结合。HNF3和GATA-4，而不是NF-1、C/EBP和GAL4-AH，在没有atp依赖酶的情况下，将它们的位点结合在致密的染色质上，并打开局部核小体结构域。HNF3打开染色质的能力是由一个高亲和力的DNA结合位点和结合组蛋白H3和H4的蛋白质c端结构域介导的。因此，在发育过程中增强转录的因子固有地能够启动染色质打开事件。
The transcription factors HNF3 (FoxA) and GATA-4 are the earliest known to bind the albumin gene enhancer in liver precursor cells in embryos. To understand how they access sites in silent chromatin, we assembled nucleosome arrays containing albumin enhancer sequences and compacted them with linker histone. HNF3 and GATA-4, but not NF-1, C/EBP, and GAL4-AH, bound their sites in compacted chromatin and opened the local nucleosomal domain in the absence of ATP-dependent enzymes. The ability of HNF3 to open chromatin is mediated by a high affinity DNA binding site and by the C-terminal domain of the protein, which binds histones H3 and H4. Thus, factors that potentiate transcription in development are inherently capable of initiating chromatin opening events.