TolC dependency of multidrug efflux systems in Salmonella enterica serovar Typhimurium
TolC dependency of multidrug efflux systems in Salmonella enterica serovar Typhimurium
复制标题
DOI：
10.1093/jac/dkq160
复制
发表时间：
2010-07-01
影响因子：
5.2
通讯作者：
Nishino, Kunihiko
中科院分区：
文献类型：
--
作者：
Horiyama, Tsukasa;Yamaguchi, Akihito;Nishino, Kunihiko
目的：TolC 是一个主要的外膜通道，在包括抗生素在内的多种分子的排泄中发挥着重要作用。最近的一项研究表明，肠沙门氏菌鼠伤寒血清型有九个功能性药物外排泵；然而，这些外排泵的 TolC 依赖性仍有待详细研究。本研究的目的是研究该生物体中多药物外排泵的 TolC 依赖性。方法：将编码药物外排系统的所有基因克隆到 pUC118 载体中。将构建的质粒转化至鼠伤寒沙门氏菌 ATCC 14028s 的 Delta acrB 和 Delta tolC 突变体中，然后测定这些转化子的药物敏感性。结果：携带 acrAB、acrD、acrEF、mdsAB、mdtABC、emrAB 或 macAB 基因的质粒不赋予 tolC 突变体抗性，而 赋予 acrB 突变体耐药性。只有三个携带 mdsABC、mdfA 或 mdtK 的质粒赋予对 tolC 突变体的抗性。结论：TolC 是鼠伤寒沙门氏菌 7 个药物流出系统（AcrAB、AcrD、AcrEF、MdsAB、MdtABC、EmrAB 和 MacAB）功能所必需的。
Objectives: TolC is a major outer membrane channel and it plays an important role in the excretion of a wide range of molecules, including antibiotics. A recent study has shown that Salmonella enterica serovar Typhimurium has nine functional drug efflux pumps; however, the TolC dependency of these efflux pumps remains to be studied in detail. The aim of this study was to investigate the TolC dependency of multidrug efflux pumps in this organism.Methods: All genes encoding the drug efflux systems were cloned into the pUC118 vector. Constructed plasmids were transformed into Delta acrB and Delta tolC mutants of S. enterica serovar Typhimurium ATCC 14028s, and then the drug susceptibilities of these transformants were determined.Results: Plasmids carrying the acrAB, acrD, acrEF, mdsAB, mdtABC, emrAB or macAB genes did not confer resistance to the tolC mutant, whereas they conferred drug resistance to the acrB mutant. Only three plasmids carrying mdsABC, mdfA or mdtK conferred resistance to the tolC mutant.Conclusions: TolC is required for the function of seven drug efflux systems (AcrAB, AcrD, AcrEF, MdsAB, MdtABC, EmrAB and MacAB) in S. enterica serovar Typhimurium.