Reaction monitoring of tocopherols with active nitrogen oxides by ultra high-speed liquid chromatography
Reaction monitoring of tocopherols with active nitrogen oxides by ultra high-speed liquid chromatography
复制标题
超高速液相色谱法监测生育酚与活性氮氧化物的反应
DOI：
10.1016/j.jpba.2010.12.036
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发表时间：
2011
影响因子：
3.4
通讯作者：
Hideko Kanazawa
中科院分区：
文献类型：
--
作者：
Yoshiko Nagata;Tadashi Nishio;Hideko Kanazawa
超高速液相色谱法（LC）在分析研究领域得到越来越广泛的应用。该分析系统在广泛的流速和压力范围内提供快速有效的色谱分离。在本研究中，我们采用超高速液相色谱系统监测α-、γ-和δ-生育酚与活性氮的反应。利用超高速液相色谱系统配以光电二极管阵列检测器，可以高效地完成对大范围反应产物的短时间分析和检测。与传统高效液相色谱法相比，生育酚及其主要氧化产物的分析时间大大缩短（10倍以上）。超反相液相色谱被证明是监测生育酚与活性氮物质快速氧化反应的有力工具。
Ultra high-speed liquid chromatography (LC) has become increasingly popular in analytical research fields. This analytical system provides fast and efficient chromatographic separation over a wide range of flow rate and pressure. In this study, we applied an ultra high-speed LC system to monitor the reaction of α-, γ-, and δ-tocopherols with active nitrogen species. By using an ultra high-speed LC system equipped with a photo-diode array detector, short time analysis and detection of a wide range of reaction products were accomplished efficiently. The analysis time of tocopherol and its major oxidation products were greatly shortened compared to conventional HPLC methods (more than 10 times). The ultra reversed-phase LC was demonstrated to be as a powerful tool for monitoring rapid oxidation reactions of tocopherols with active nitrogen species.