PHYLOGENETIC DIVERSITY OF THE BACTERIAL COMMUNITY FROM A MICROBIAL MAT AT AN ACTIVE, HYDROTHERMAL VENT SYSTEM, LOIHI SEAMOUNT, HAWAII
PHYLOGENETIC DIVERSITY OF THE BACTERIAL COMMUNITY FROM A MICROBIAL MAT AT AN ACTIVE, HYDROTHERMAL VENT SYSTEM, LOIHI SEAMOUNT, HAWAII
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DOI：
10.1128/aem.61.4.1555-1562.1995
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发表时间：
1995-04-01
影响因子：
4.4
通讯作者：
KARL, DM
中科院分区：
文献类型：
--
作者：
MOYER, CL;DOBBS, FC;KARL, DM
研究了与细菌结构域相关的小亚基rRNA基因的系统发育多样性（通过使用先前定义的操作分类单位）[C]。L.莫耶，F. C.多布斯和D. M.卡尔，苹果。环绕。微生物学通报，2009；贝利热液喷口细菌（以下简称PVB OTUs）的样本来自活跃的深海热液喷口系统的微生物席。一个系统发育相关的PVB otu群（otu 2、3、6和8）与Proteobacteria类epsilon亚类中的Thiovulum sp.密切相关，占Pele’s Vents小亚基rRNA细菌克隆文库的60.5%。第二个较小的PVB OTUs簇（OTUs 1和11）与变形菌门γ亚纲中的黄单胞菌密切相关，占细菌克隆文库的27.1%。其余5个PVB OTU各占恢复克隆的2.1%，隶属于以下系统发育类群：PVB OTU 5属于Alteromonas类群；PVB OTU 12是Colwellia集合的成员；PVB OTU 4与嗜热深海菌（Bathymodiolus thermoophilus）和巨型Calyptogena为内共生菌，大致确定为硫思菌属；PVB OTU 10B属黏菌群；PVB OTU 9A是副葡萄组合的成员，与Octopus Spring微生物席型K克隆是已知的最近的亲缘关系。PVB OTU 7被确定为pcr生成的嵌合结构，结合了本研究中检测到的两种描述的种型，从而将先前估计的PVB主要OTU的数量从12个减少到11个。
The phylogenetic diversity of small-subunit rRNA genes associated,vith the domain Bacteria was examined (by using previously defined operational taxonomic units [C. L. Moyer, F. C. Dobbs, and D. M. Karl, Appl. Environ. Microbiol. 60:871-879, 1994]; those for Pele's Vents Bacteria are hereafter abbreviated PVB OTUs) with samples from a microbial mat at an active, deep-sea hydrothermal vent system. A cluster of phylogenetically related PVB OTUs (OTUs 2, 3, 6, and 8) was closely affiliated with Thiovulum sp. contained within the epsilon subclass of the class Proteobacteria and accounted for 60.5% of the small-subunit rRNA bacterial clone library from Pele's Vents. A second, smaller cluster of PVB OTUs (OTUs 1 and 11) was closely affiliated with Xanthomonas sp., contained within the gamma subclass of the Proteobacteria and accounted for a total of 27.1% of the bacterial clone library. The remaining five PVB OTUs each accounted for 2.1% of the clones recovered and were affiliated with the following phylogenetic groups: PVB OTU 5 was a member of the Alteromonas group; PVB OTU 12 was a member of the Colwellia assemblage; PVB OTU 4 was loosely determined to be a member of the Thiothrix group, with the endosymbiotic bacteria from Bathymodiolus thermophilus and Calyptogena magnifica as the nearest relatives; PVB OTU 10B was a member of the Myxobacterium group; and PVB OTU 9A was a member of the Paraphyletic assemblage, with the Octopus Spring microbial mat type K clone as the closest known relative. PVB OTU 7 was determined to be a PCR-generated chimeric structure combined from two described phylotypes detected in this study, thereby decreasing the previously estimated number of major PVB OTUs from 12 to 11.