Preparation and characterization of superparamagnetic iron oxide nanoparticles stabilized by alginate
Preparation and characterization of superparamagnetic iron oxide nanoparticles stabilized by alginate
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DOI：
10.1016/j.ijpharm.2006.10.006
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发表时间：
2007-03-21
影响因子：
5.8
通讯作者：
Nagai, Tsuneji
中科院分区：
文献类型：
--
作者：
Ma, Hui-li;Qi, Xian-tong;Nagai, Tsuneji
具有适当表面化学性质的SPION已在实验上广泛用于许多体内应用。本文采用改进的共沉淀法制备了海藻酸钠稳定的SPION（SPION-海藻酸钠）。采用XRD、TEM、ESEM、AFM、DJLS、SQUID磁强计和MRI等手段对SPION-海藻酸钠的结构、尺寸、形貌、磁性和弛豫率进行了系统表征，并采用FT-IR和AFM表征了海藻酸钠与氧化铁（Fe 3 O 4）的相互作用。结果表明，典型的氧化铁纳米粒子为Fe_3O_4，核直径为5-10 nm，SPION-海藻酸钠的流体动力学直径为193.8-483.2 nm。由磁化曲线可知，SPION-藻酸盐悬浮液的Ms为40 emu/g，相当于固体SPION-藻酸盐的73%。这种高Ms可能是由于Fe 3 O 4纳米颗粒与藻酸盐大分子链的结合，如通过AFM视觉确认的。SPION-藻酸盐的几百纳米是稳定的大小为12个月在4摄氏度。此外，SPION-藻酸盐在盐水（1.5 T，20 ℃）中的T1弛豫率和T2弛豫率分别为7.86 +/- 0.20 s（-1）mM（-1）和281.2 +/- 26.4 s（-1）mM（-1）。(c)2006 Elsevier B. V.保留所有权利。
SPION with appropriate surface chemistry have been widely used experimentally for numerous in vivo applications. In this study, SPION stabilized by alginate (SPION-alginate) were prepared by a modified coprecipitation method. The structure, size, morphology, magnetic property and relaxivity of the SPION-alginate were characterized systematically by means of XRD, TEM, ESEM, AFM, DJLS, SQUID magnetometer and MRI, respectively, and the interaction between alginate and iron oxide (Fe3O4) was characterized by FT-IR and AFM. The results revealed that typical iron oxide nanoparticles were Fe3O4 With a core diameter of 5-10 nm and SPION-alginate had a hydrodynamic diameter of 193.8-483.2 nm. From the magnetization curve, the Ms of a suspension of SPION-alginate was 40 emu/g, corresponding to 73% of that of solid SPION-alginate. This high Ms may be due to the binding of Fe3O4 nanoparticles to alginate macromolecule strands as visually confirmed by AFM. SPION-alginate of several hundred nanometers was stable in size for 12 months at 4 degrees C. Moreover, T1 relaxivity and T2 relaxivity of SPION-alginate in saline (1.5 T, 20 degrees C) were 7.86 +/- 0.20 s(-1) mM(-1) and 281.2 +/- 26.4 s(-1) mM(-1), respectively. (c) 2006 Elsevier B.V. All rights reserved.