Characterization, and comparison, of human clinical and black-headed gull (Larus ridibundus) extended-spectrum β-lactamase-producing bacterial isolates from Kalmar, on the southeast coast of Sweden
Characterization, and comparison, of human clinical and black-headed gull (Larus ridibundus) extended-spectrum β-lactamase-producing bacterial isolates from Kalmar, on the southeast coast of Sweden
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DOI：
10.1093/jac/dkq222
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发表时间：
2010-09-01
影响因子：
5.2
通讯作者：
Drobni, M.
中科院分区：
文献类型：
--
作者：
Bonnedahl, J.;Drobni, P.;Drobni, M.
抗生素耐药性是现代医疗保健面临的重大挑战之一。在革兰氏阴性菌中，CTX-M型超广谱β-内酰胺酶（ESBLs）自21世纪初以来在欧洲迅速蔓延。在瑞典，产ESBL大肠杆菌仍然很罕见，但从2004年到2007年增加了3倍。肠杆菌和野生动物的正常植物群，具有或不具有抗生素抗性特征，构成人类感染和定殖的潜在来源。我们研究了野生鸟类，目的是了解抗生素耐药性的环境传播，重点是临床相关的耐药类型，我们与人类临床样本进行了比较。在这项研究中，产ESBL的人类临床分离株和来自瑞典东南海岸卡尔马县医院和卡尔马市的青少年黑头鸥的分离株，产ESBL大肠杆菌的耐药率虽然较低，但其耐药率仍高于其他菌株。共检出10株大肠杆菌，其中2株（CTX-M-14）为bla，1株（CTX-M-15）为bla。ESBL分离株以CTX-M型为主。此外，在PhenePlate（TM）聚类系统中，海鸥分离株分散在人群中，表明它们与人群分离株没有区别，也没有形成任何单独的克隆聚类。从瑞典的黑头鸥中分离出的大肠杆菌，“背景抗性”很低，这与这些质粒携带的抗性基因正在进行的环境传播是一致的。结果表明，即使在抗生素耐药性水平较低的国家，也存在人口与环境之间转移的可能性。
Antibiotic resistance is one of the great challenges for modern healthcare. In Gram-negative bacteria, CTX-M-type extended-spectrum beta-lactamases (ESBLs) have been rapidly spreading through Europe since the early 2000s. In Sweden, ESBL-producing Escherichia coli are still rare, but a 3-fold increase has been seen from 2004 to 2007. Enterobacteria and normal flora of wild animals, with or without antibiotic resistance traits, constitute a potential source of human infection and colonization. We studied wild birds with the aim to understand the environmental dissemination of antibiotic resistance and, focusing on clinically relevant resistance types, we made comparisons with human clinical samples.In this study, ESBL-producing human clinical isolates and isolates from juvenile black-headed gulls from Kalmar County hospital and the city of Kalmar, respectively, on the southeast coast of Sweden, were characterized and compared.Despite a low frequency of antibiotic resistance among the isolates from gulls, ESBL-producing E. coli isolates were found, two with bla(CTX-M-14) and one with bla(CTX-M-15). The same CTX-M types were dominant among human ESBL isolates. In addition, gull isolates were dispersed among the human samples in the PhenePlate (TM) clustering system, indicating that they neither differ from the human isolates nor form any separate clonal clustering.The finding of CTX-M-type ESBLs in E. coli isolated from black-headed gulls in Sweden, where 'background resistance' is low, is consistent with an ongoing environmental spread of these plasmid-borne resistance genes. The results indicate that a potential for transfer between the human population and environment exists even in countries with a low level of antibiotic resistance.