Detection of the differentially expressed gene IGF-binding protein-related protein-1 and analysis of its relationship to fasting glucose in Chinese colorectal cancer patients
Detection of the differentially expressed gene IGF-binding protein-related protein-1 and analysis of its relationship to fasting glucose in Chinese colorectal cancer patients
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DOI：
10.1677/erc.0.0110141
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发表时间：
2004-03-01
影响因子：
3.9
通讯作者：
Lai, M
中科院分区：
文献类型：
--
作者：
Shao, L;Huang, Q;Lai, M
IGF结合蛋白相关蛋白1（IGFBP-rP 1）是IGF轴的成员。在我们以前的工作中，我们从结肠腺癌和正常粘膜cIDNA消减文库中分离了IGFBP-rP 1的cDNA片段。本研究通过半定量RTPCR和免疫组化方法比较IGFBP-rP 1在大肠癌、腺瘤、癌旁正常组织和正常组织中的表达。进一步探讨IGFBP-rP 1与血糖之间的关系。IGFBP-rP 1 mRNA在胃癌组织中的表达最高，在腺瘤和癌旁组织中的表达次之，在正常组织中的表达最低（P < 0.05）。IGFBP-rP 1在癌组织和癌旁正常组织中的表达差异有统计学意义（P < 0.05）。IGFBP-rP 1表达上调的肿瘤组织中，淋巴结转移率、浸润深度和IGFBP-rP 1染色强度均高于其他组织（P < 0.05）。空腹血糖水平与IGFBP-rP 1在癌组织中的表达呈正相关（斯皮尔曼氏rho = 0.4，P < 0.000）。因此，我们认为IGFBP-rP 1过表达可能在大肠癌的发生和发展中起重要作用。IGFBP-rP 1的表达也可能与空腹血糖水平和糖尿病的存在有关。
IGF-binding protein-related protein-1 (IGFBP-rP1) is a member of the IGF axis. In our previous work, we separated cDNA fragments of IGFBP-rP1 from colonic adenocarcinoma and normal mucosa cIDNA subtraction libraries. In this study, we compared the expression of IGFBP-rP1 by semi-quantitative RTPCR and immunohistochemistry among colorectal cancer, adenoma, normal tissue adjacent to cancer site, and normal tissue. Associations between IGFBP-rP1 and plasma glucose were further explored. We found that the mRNA level of IGFBP-rP1 was highest in cancer, moderate in adenoma and tissue adjacent to the cancer site and lowest in normal tissue (P < 0.05). A significant difference was found in the immunoreactivity of IGFBP-rP1 between paired normal and cancer tissue (P < 0.05). Tumor samples with upregulated expression of IGFBP-rP1 in invading tumor cells showed an increased frequency of metastasis to the lymph node, an increased depth of infiltration and stronger staining of IGFBP-rP1 compared with other samples (P < 0.05). The fasting glucose level was significantly correlated with the staining of IGFBP-rP1 in cancer tissue (Spearman's rho = 0.4, P < 0.000). Thus, we concluded overexpression of IGFBP-rP1 might play an important role in the initiation and promotion of colorectal cancer. IGFBP-rP1 expression may also be associated with fasting glucose level and the presence of diabetes mellitus.