Early detection of myocardial infarction in conscious dogs by analysis of plasma MM creatine kinase isoforms.
Early detection of myocardial infarction in conscious dogs by analysis of plasma MM creatine kinase isoforms.
复制标题
通过分析血浆 MM 肌酸激酶亚型早期检测清醒犬的心肌梗塞。
DOI：
10.1161/01.cir.71.2.363
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发表时间：
1985
期刊：
影响因子：
37.8
通讯作者：
Sobel,BE
中科院分区：
文献类型：
--
作者：
Hashimoto,H;Abendschein,DR;Strauss,AW;Sobel,BE
为了确定是否可以通过分析血浆中肌酸激酶 (MM CK) MM 同工酶 (亚型) 的亚型来确定是否可以在发病后早期检测到心肌梗死，我们对八只清醒的狗进行冠状动脉闭塞，并通过色谱聚焦对连续血浆样本中的亚型进行定量。冠状动脉闭塞前血浆样本中MMA（等电点[pI] = 7.91）、MMB（pI = 7.74）和MMC（pI = 7.51）的分数平均占总MM CK活性的11.4 +/- 4.8% (SD)、22.3 +/- 5.5%和66.3 +/- 9.6%。冠状动脉闭塞后 1 小时，血浆中 MMA 的分数（心肌中发现的 MM CK 亚型）显着增加，在 4.1 +/- 1.3 小时内达到最大值 49.7 +/- 8.0%，并在 12.0 +/- 2.3 小时内恢复到基线。归因于 MMC 的血浆 MM CK 活性分数，MMC 是一种通过 MMB 作为中间体在血浆中缓慢形成的同种型，在 1 小时内显着下降，在 4.8 +/- 1.1 小时内达到最低 14.0 +/- 4.1%，并在冠状动脉闭塞后 13.0 +/- 2.9 小时内恢复到基线。总 CK 活性直到后来（即闭塞后 5 小时）才显着增加，并在 10.9 +/- 1.9 小时内达到峰值 1371 +/- 530 IU/升。在冠状动脉闭塞后的前 4 小时内，MMA 始终占血浆 MM CK 活性的 20% 以上，尽管总 CK 增加不显着。同种型比例的变化是一致的，且独立于总 CK 活性峰值和 10 倍范围内的累积 CK 释放。因此，通过血浆 MM CK 同种型的定量，可在缺血发作后 1 小时内检测到指示梗塞的初始 CK 释放。
To determine whether myocardial infarction could be detected early after onset by analysis of subforms of the MM isoenzyme (isoforms) of creatine kinase (MM CK) in plasma, we subjected eight conscious dogs to coronary occlusion and quantified isoforms in serial plasma samples by chromatofocusing. The fractions of MMA (isoelectric point [pI] = 7.91), MMB (pI = 7.74), and MMC (pI = 7.51) in plasma samples before coronary occlusion averaged 11.4 +/- 4.8% (SD), 22.3 +/- 5.5%, and 66.3 +/- 9.6% of total MM CK activity. The fraction of MMA, the isoform of MM CK found in myocardium, increased significantly in plasma 1 hr after coronary occlusion, reached a maximum of 49.7 +/- 8.0% in 4.1 +/- 1.3 hr, and returned to baseline in 12.0 +/- 2.3 hr. The fraction of plasma MM CK activity attributable to MMC, an isoform formed slowly in plasma from MMA via MMB as an intermediate, decreased significantly within 1 hr, reached a minimum of 14.0 +/- 4.1% in 4.8 +/- 1.1 hr, and returned to baseline in 13.0 +/- 2.9 hr after coronary occlusion. Total CK activity did not increase significantly until later, i.e., 5 hr after occlusion, and peaked at 1371 +/- 530 IU/liter in 10.9 +/- 1.9 hr. Within the first 4 hr after coronary occlusion, MMA consistently comprised more than 20% of plasma MM CK activity despite insignificant increase of total CK. Changes in isoform proportions were consistent and independent of peak total CK activity and of cumulative CK release over a 10-fold range. Thus initial CK release indicative of infarction is detectable within 1 hr after the onset of ischemia by quantification of plasma MM CK isoforms.