Albumin storage in neoplastic astroglial elements of gangliogliomas.
Albumin storage in neoplastic astroglial elements of gangliogliomas.
复制标题
神经节胶质瘤的肿瘤性星形胶质细胞中的白蛋白储存。
DOI：
10.1016/j.seizure.2012.10.014
复制
发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Niehusmann,Pitt
中科院分区：
文献类型：
--
作者：
Schmitz,AnnKristin;Grote,Alexander;Raabe,Anna;Urbach,Horst;Friedman,Alon;vonLehe,Marec;Becker,AlbertJ;Niehusmann,Pitt
低级别神经上皮肿瘤是耐药性癫痫患者常见的神经病理学表现。关于这组肿瘤的致癫痫机制知之甚少，其中神经节细胞胶质瘤（GG）是最常见的肿瘤。GG中含铁血黄素沉积物的存在表明血脑屏障（BBB）受损。因此，我们推测血脑屏障功能障碍和星形胶质细胞白蛋白摄取可能是GG的潜在致痫因素。方法采用普鲁士蓝染色和免疫荧光双标法检测GG（n=10）和几个对照组的肿瘤和邻近脑组织中含铁血黄素沉积和星形胶质细胞白蛋白积聚，即胚胎发育不良性神经上皮肿瘤（DNT; n=5）、具有气球状细胞的局灶性皮质发育不良（FCD IIb; n=10）、星形细胞瘤WHO II级（n=5）和透明肾细胞癌脑转移（RCCM，n=6）。有趣的是，我们注意到大量的白蛋白摄取专门在肿瘤性胶质细胞成分的GG和DNT，而没有显着的白蛋白存在于病灶周围的反应性星形胶质细胞。引人注目的是，我们没有观察到大量的白蛋白摄取进一步controls. CONCLUSIONLIAL白蛋白摄取局限于长期癫痫相关，含血管的肿瘤。肿瘤内血脑屏障功能障碍与肿瘤性胶质细胞成分引起的白蛋白蓄积共同代表了长期癫痫相关肿瘤的一种新的致癫痫机制。
PURPOSELow-grade neuroepithelial tumors are frequent neuropathological findings in patients with pharmacoresistant epilepsies. Little is known regarding epileptogenic mechanisms in this group of neoplasms with gangliogliomas (GG) as the most common entity. Presence of hemosiderin deposits in GG points to impairment of the blood-brain barrier (BBB). Therefore, we hypothesized a potential role of BBB dysfunction and astrocytic albumin uptake as potential epileptogenic factor in GG.METHODSPrussian blue staining and fluorescent double-immunohistochemistry with antibodies against albumin, GFAP, CD34 and GLUT-1 were used to analyze hemosiderin deposits and astroglial albumin accumulation in tumor and adjacent pre-existing brain tissue of GG (n=10) and several control groups, i.e. dysembryoplastic neuroepithelial tumors (DNT; n=5), focal cortical dysplasia with balloon cells (FCD IIb; n=10), astrocytomas WHO grade II (n=5) and clear renal cell carcinoma brain metastases (RCCM, n=6).RESULTSOur results revealed strong hemosiderin deposits in GG. Intriguingly, we noted substantial albumin uptake exclusively in neoplastic glial cell components of GG and DNT, whereas no significant albumin was present in perilesional reactive astrocytes. Strikingly, we did not observe substantial albumin uptake in further controls.CONCLUSIONGlial albumin uptake was restricted to long-term epilepsy associated, vasculature-containing tumors. Intratumoural BBB dysfunction in concert with subsequent accumulation of albumin by neoplastic glial cell elements represent a new putatively epileptogenic mechanism for long-term epilepsy-associated tumors.