Synergistic effects of phenolics and carotenoids on human low-density lipoprotein oxidation
Synergistic effects of phenolics and carotenoids on human low-density lipoprotein oxidation
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DOI：
10.1002/mnfr.200600271
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发表时间：
2007-08-01
影响因子：
5.2
通讯作者：
Grabmann, Johanna
中科院分区：
文献类型：
--
作者：
Mildel, Jens;Elstner, Erich F.;Grabmann, Johanna
低密度脂蛋白氧化被认为在动脉粥样硬化的发展中起重要作用，因此LDL对氧化的高抗性可以防止动脉粥样硬化形成和伴随的疾病。已经测试了几种次生植物代谢物防止LDL氧化的能力，并且许多酚类物质以及类胡萝卜素已经显示出增强LDL抗氧化性。我们发现槲皮苷芦丁能够抑制铜诱导的LDL中共轭二烯的形成和色氨酸荧光的丧失。然而，富含类胡萝卜素叶黄素或番茄红素的LDL并没有导致LDL氧化的减轻。由于人们一致认为，没有一种抗氧化剂单独可以带来健康益处，而是组合，例如在水果和蔬菜中发现的组合，是有效成分，我们测试了酚类化合物的组合（即，e.芦丁）和类胡萝卜素（即叶黄素或番茄红素）导致协同效应。这两种组合显示出对LDL的氧化发挥超累加保护作用，这很可能是由于LDL颗粒中抗氧化剂的不同分配和抗氧化作用的不同机制。
Low-density lipoprotein oxidation is believed to play an important role in the development of atherosclerosis and therefore a high resistance of LDL against oxidation may prevent atherogenesis and accompanying disorders. Several secondary plant metabolites have been tested for their ability to prevent oxidation of LDL and many phenolics as well as carotenoids have been shown to enhance LDL oxidation resistance. We showed that the quercetingylcoside rutin is able to inhibit copper-induced formation of conjugated dienes and loss of tryptophan fluorescence in LDL. However, enrichment of LDL with the carotenoids lutein or lycopene did not result in an alleviation of LDL oxidation. Since there is an agreement that not one antioxidant alone can lead to health benefits but the combination, as found for example in fruits and vegetables, is the active principle, we tested whether the combination of a phenolic compound (i. e. rutin) and carotenoids (i.e. lutein or lycopene) leads to synergistic effects. Both combinations were shown to exert supra-additive protection of LDL towards oxidation, which is most likely due to different allocation of the antioxidants in the LDL-particle and to different mechanisms of antioxidam action.