The characteristics of stem cells in human degenerative intervertebral disc.
The characteristics of stem cells in human degenerative intervertebral disc.
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人类退变椎间盘干细胞的特性
DOI：
10.1097/md.0000000000007178
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发表时间：
2017-06
期刊：
影响因子：
1.6
通讯作者：
Zhu Y
中科院分区：
文献类型：
--
作者：
Liang L;Li X;Li D;Jiang W;Wang H;Chen J;Sun Z;Zhang N;Zhu Y
背景：本研究的目的是确定在人类退行性椎间盘的3种细胞中，哪一种具有最好的干细胞样特征：NPSCs（髓核来源的干细胞）、AFSCs（纤维环来源的干细胞）和CESCs（软骨终板来源的干细胞）。方法：我们将15例椎间盘退行性疾病手术患者的椎间盘标本分为髓核、纤维环和软骨终板。培养后，使用细胞计数试剂盒-8分别分析3种盘状组织源性干细胞的细胞活性；不同的干细胞被定义为多谱系（成骨、软骨和脂肪）分化。我们提取总RNA，用实时聚合酶链反应（RT-PCR）检测不同谱系分化相关基因的表达。结果：NPSCs、AFSCs和CESCs的细胞形态无明显差异。NPSCs和AFSCs的细胞增殖能力强于CESCs。分化结果显示，茜素红S将骨细胞样细胞染成红色，甲苯胺蓝将软骨细胞样细胞染成蓝色，油红o将脂肪细胞样细胞染成红色。RT-PCR反映AFSCs不同谱系分化相关基因的表达强于NPSCs和CESCs。结论：综上所述，我们发现NPSCs、AFSCs和CESCs之间的细胞形态没有显著差异。分化和RT-PCR试验均表明，AFSCs在人类退行性椎间盘中具有最佳的干细胞样特征。
Background: The aim of this study is to identify which possessed the best stem-cell-like characteristics in 3 kinds of cell in human degenerative intervertebral disc: NPSCs (nucleus pulposus-derived stem cells), AFSCs (annulus fibrosus-derived stem cells), or CESCs (cartilage endplate-derived stem cells). Methods: We separated the disc samples obtained from 15 surgically treated patients with disc degenerative diseases into nucleus pulposus, annulus fibrosus, and cartilage endplate. After cultivating, we used the cell counting kit-8 to analysis the cell activity of 3 kinds of disc tissue-derived stem cell separately; different stem cells were defined with multilineage (osteogenic, chondrogenic, and adipogenic) differentiation. We extracted the total RNA and detected the expression of different lineage differentiation-related genes with the real-time polymerase chain reaction (RT-PCR). Results: Cell morphology of NPSCs, AFSCs, and CESCs did not show significant difference. Cell proliferation capacity of NPSCs and AFSCs was stronger than that of CESCs. The differentiation outcomes showed that osteocyte-like cells were stained red by Alizarin red S, chondrocyte-like cells blue by toluidine blue, and adipocyte-like red by oil red O. The RT-PCR reflected that the expression of different lineage differentiation-related genes of AFSCs was stronger than NPSCs and CESCs. Conclusion: In conclusion, we found that the cell morphology was not significantly different among NPSCs, AFSCs, and CESCs. Both differentiation and RT-PCR tests demonstrated that AFSCs had the best stem-cell-like characteristics in the human degenerative intervertebral disc.