Endogenous Regulatory T Cells Promote M2 Macrophage Phenotype in Diabetic Stroke as Visualized by Optical Imaging
Endogenous Regulatory T Cells Promote M2 Macrophage Phenotype in Diabetic Stroke as Visualized by Optical Imaging
复制标题
光学成像显示内源性调节性 T 细胞促进糖尿病中风中的 M2 巨噬细胞表型
DOI：
10.1007/s12975-020-00808-x
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发表时间：
2020-04-02
影响因子：
6.9
通讯作者：
Ju, Shenghong
中科院分区：
文献类型：
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作者：
Cai, Yu;Xu, Ting-Ting;Ju, Shenghong
调节性T细胞（Regulatory T cells，TCFs）在多种疾病中发挥免疫抑制作用，但其在脑卒中中的作用仍存在争议，在糖尿病脑卒中中的作用也不明确。在本研究中，发现与对照组相比，2型糖尿病（T2 DM）脑卒中模型和患者外周血中的TlR表达下调。在缺血性中风小鼠（T2 DM和野生型）中，通过CD 28 SA增强的内源性Tcl 3增加了CD 206 + M2巨噬细胞/小胶质细胞，减少了梗死体积，并改善了神经恢复。我们的结果证明了提高TdR治疗T2 DM卒中的潜力。此外，我们利用光学成像探针（IRD-α CD 206）靶向M2巨噬细胞/小胶质细胞，并证明其在缺血脑组织中体内可视化M2巨噬细胞/小胶质细胞的效果。
Regulatory T cells (Tregs) play an immunosuppressive role in various diseases, yet their function remains controversial in stroke and obscure in diabetic stroke. In the present study, Tregs were found downregulated in the peripheral blood of type 2 diabetes mellitus (T2DM) stroke models and patients compared with controls. In ischemic stroke mice (both T2DM and wild type), endogenous Tregs boosted by CD28SA increased CD206+ M2 macrophage/microglia cells, decreased infarct volumes, and improved neurological recovery. Our results demonstrated the potential of boosting Tregs for treating T2DM stroke. Furthermore, we utilized an optical imaging probe (IRD-αCD206) to target M2 macrophage/microglia cells and demonstrated its effect in visualizing M2 macrophage/microglia cells in vivo in ischemic brain tissue.