The zinc finger protein DCM1 is required for male meiotic cytokinesis by preserving callose in rice.
The zinc finger protein DCM1 is required for male meiotic cytokinesis by preserving callose in rice.
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通过保存水稻中的胼胝质，锌指蛋白 DCM1 是雄性减数分裂胞质分裂所必需的
DOI：
10.1371/journal.pgen.1007769
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发表时间：
2018-11
期刊：
影响因子：
4.5
通讯作者：
Cheng Z
中科院分区：
文献类型：
--
作者：
Zhang C;Shen Y;Tang D;Shi W;Zhang D;Du G;Zhou Y;Liang G;Li Y;Cheng Z
减数分裂影响配子的育性和倍性。然而，有限的信息是在植物减数分裂胞质分裂的遗传控制。在这里，我们确定了一个水稻突变体低雄性育性，缺陷胼胝质减数分裂1（dcm 1）。dcm 1的花粉外壁形成缺陷。减数分裂胞质分裂在dcm 1中被破坏，导致减数分裂II期间纺锤体方向紊乱，并形成具有不同大小和DNA含量的花粉粒。我们证明dcm 1的减数分裂胞质分裂缺陷是由胼胝质板过早溶解引起的。此外，dcm 1花粉母细胞（PMC）周围的周边胼胝质也消失了不合时宜的粗线期。DCM 1蛋白含有5个串联的CCCH基序，并在核斑点中与核多聚腺苷酸结合蛋白（PABN）相互作用。与胼胝质合成和降解相关基因的表达谱在dcm 1中被显著改变。总之，我们建议，DCM 1起着至关重要的作用，在男性减数分裂胞质分裂保存胼胝质过早溶解在水稻。减数分裂包括单个S期后两次连续的细胞分裂，产生四个单倍体产物。减数分裂有核分裂（核分裂）已被广泛研究，在许多生物，而减数分裂胞质分裂的机制仍然难以捉摸。在这里，我们确定了一种新的CCCH串联锌指蛋白DCM 1，防止过早溶解胼胝质周围的PMC和分裂网站（胼胝质板）。胼胝质板的丢失破坏了减数分裂的胞质分裂，导致减数分裂II期间纺锤体的随机分布和异常的减数分裂产物。DCM1与两个水稻poly（A）结合蛋白相互作用，独立于保守的CCCH结构域。此外，DCM1协调胼胝质合成和降解相关基因的表达谱。我们推测单子叶植物和双子叶植物在雄配子产生过程中可能采用不同的减数分裂胞质分裂模式。
Meiotic cytokinesis influences the fertility and ploidy of gametes. However, limited information is available on the genetic control of meiotic cytokinesis in plants. Here, we identified a rice mutant with low male fertility, defective callose in meiosis 1 (dcm1). The pollen grains of dcm1 are proved to be defective in exine formation. Meiotic cytokinesis is disrupted in dcm1, resulting in disordered spindle orientation during meiosis II and formation of pollen grains with varied size and DNA content. We demonstrated that meiotic cytokinesis defect in dcm1 is caused by prematurely dissolution of callosic plates. Furthermore, peripheral callose surrounding the dcm1 pollen mother cells (PMCs) also disappeared untimely around pachytene. The DCM1 protein contains five tandem CCCH motifs and interacts with nuclear poly (A) binding proteins (PABNs) in nuclear speckles. The expression profiles of genes related to callose synthesis and degradation are significantly modified in dcm1. Together, we propose that DCM1 plays an essential role in male meiotic cytokinesis by preserving callose from prematurely dissolution in rice. Meiosis comprises two successive cell divisions after a single S phase, generating four haploid products. Meiotic caryokinesis (nuclear division) has been extensively studied in many organisms, while mechanisms underlying meiotic cytokinesis remain elusive. Here, we identified a novel CCCH-tandem zinc finger protein DCM1 that prevent the premature dissolution of callose both around the PMCs and at the dividing site (callosic plates). Loss of the callosic plates disrupts the meiotic cytokinesis, leading to the random distribution of spindles during meiosis II and aberrant meiotic products. DCM1 interacts with the two rice poly (A) binding proteins, independently of the conserved CCCH domain. Moreover, DCM1 coordinates the expression profiles of genes related to callose synthesis and degradation. We suspect monocots and dicots may adopt distinct meiotic cytokinesis patterns during male gamete generation.
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影响因子：
16
作者：
Lemieux, Caroline;Marguerat, Samuel;Bachand, Francois
通讯作者：
Bachand, Francois
DOI：
10.1261/rna.055608.115
发表时间：
2016-09
期刊：
RNA (New York, N.Y.)
影响因子：
--
作者：
Marayati BF;Hoskins V;Boger RW;Tucker JF;Fishman ES;Bray AS;Zhang K
通讯作者：
Zhang K
影响因子：
2.9
作者：
De Storme N;Geelen D
通讯作者：
Geelen D
DOI：
10.1083/jcb.200811072
发表时间：
2009-04-20
期刊：
The Journal of cell biology
影响因子：
--
作者：
Hurt JA;Obar RA;Zhai B;Farny NG;Gygi SP;Silver PA
通讯作者：
Silver PA
DOI：
10.1261/rna.044479.114
发表时间：
2014-06
期刊：
RNA (New York, N.Y.)
影响因子：
--
作者：
Egan ED;Braun CR;Gygi SP;Moazed D
通讯作者：
Moazed D