Receptor-driven, multimodal mapping of the human amygdala
Receptor-driven, multimodal mapping of the human amygdala
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DOI：
10.1007/s00429-017-1577-x
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发表时间：
2018-05-01
影响因子：
3.1
通讯作者：
Amunts, Katrin
中科院分区：
文献类型：
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作者：
Kedo, Olga;Zilles, Karl;Amunts, Katrin
人类的杏仁核由具有不同功能的部分组成。然而，在细胞和分子水平上的结构组织原理还没有被很好地理解。因此，我们重新分析了杏仁核的细胞结构，并基于新的工作流程和映射工具生成了立体定位空间中十个细分的细胞结构概率图。这部分随后被用作分析15种受体类型的受体表达的基础。受体指纹，即所有受体类型的密度之间的特征平衡，在每个细分中生成，以全面可视化不同细分之间受体结构的差异和相似之处。中央、内侧核和杏仁前区的指纹高度相似。外侧核、基底外侧核和基底内侧核的指纹图谱也相似，其余核团的指纹图谱形状不同。通过等级聚类分析进一步研究了相似性：一个两个簇的解决方案将系统发育较老的部分(中央、内侧核、杏仁前区)从杏仁核的其余部分细分出来。一个更细粒度的三簇解决方案复制了我们之前的分离，包括基底侧、浅层和中央内侧组。此外，它有助于更好地描述板旁核，在基底侧和浅层之间有一个分子组织。人类杏仁核的多模式细胞和受体构筑分析为其微观结构组织、受试者之间的变异性、立体定位空间的定位以及基于受体的神经化学差异提供了新的见解。
The human amygdala consists of subdivisions contributing to various functions. However, principles of structural organization at the cellular and molecular level are not well understood. Thus, we re-analyzed the cytoarchitecture of the amygdala and generated cytoarchitectonic probabilistic maps of ten subdivisions in stereotaxic space based on novel workflows and mapping tools. This parcellation was then used as a basis for analyzing the receptor expression for 15 receptor types. Receptor fingerprints, i.e., the characteristic balance between densities of all receptor types, were generated in each subdivision to comprehensively visualize differences and similarities in receptor architecture between the subdivisions. Fingerprints of the central and medial nuclei and the anterior amygdaloid area were highly similar. Fingerprints of the lateral, basolateral and basomedial nuclei were also similar to each other, while those of the remaining nuclei were distinct in shape. Similarities were further investigated by a hierarchical cluster analysis: a two-cluster solution subdivided the phylogenetically older part (central, medial nuclei, anterior amygdaloid area) from the remaining parts of the amygdala. A more fine-grained three-cluster solution replicated our previous parcellation including a laterobasal, superficial and centromedial group. Furthermore, it helped to better characterize the paralaminar nucleus with a molecular organization in-between the laterobasal and the superficial group. The multimodal cyto- and receptor-architectonic analysis of the human amygdala provides new insights into its microstructural organization, intersubject variability, localization in stereotaxic space and principles of receptor-based neurochemical differences.