The Zinc Finger Transcription Factor SlZFP2 Negatively Regulates Abscisic Acid Biosynthesis and Fruit Ripening in Tomato1
The Zinc Finger Transcription Factor SlZFP2 Negatively Regulates Abscisic Acid Biosynthesis and Fruit Ripening in Tomato1
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DOI：
10.1104/pp.114.255174
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发表时间：
2015-01
期刊：
影响因子：
7.4
通讯作者：
Lin Weng;Fangfang Zhao;Rong Li;Changjie Xu;Kun-song Chen;Han Xiao
中科院分区：
文献类型：
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作者：
Lin Weng;Fangfang Zhao;Rong Li;Changjie Xu;Kun-song Chen;Han Xiao
一种抑制脱落酸生物合成和果实成熟的转录因子。脱落酸（阿坝）调节植物的发育和对环境条件的适应。虽然植物中阿坝生物合成途径已被彻底阐明，但阿坝生物合成基因在植物发育过程中如何在分子水平上受到调控还不太清楚。在这里，我们表明，番茄（Solanum lycopersicum）锌指转录因子SlZFP 2参与调节果实发育过程中的阿坝生物合成。SlZFP 2的过表达导致多个表型变化，包括更多的分枝、提早开花、延迟果实成熟、更轻的种子和更快的种子萌发，而其表达的下调导致有问题的坐果、加速成熟和抑制种子萌发。SlZFP 2通过直接抑制阿坝生物合成基因NOTABILIS、SITIENS和FLACA以及醛氧化酶SlAO 1来抑制果实发育期间的阿坝生物合成。我们还表明，SlZFP 2通过转录抑制成熟调节子COLORLESS NON-RIPENING来调节果实成熟。使用细菌单杂交筛选和选择的扩增和结合测定，我们鉴定了（A/T）（G/C）TT基序作为SlZFP 2的核心结合序列。此外，通过RNA测序分析，我们发现193个基因的启动子中含有SlZFP 2结合基序在开花后2天的果实之间的SlZFP 2 RNA干扰线和它的非转基因同胞的差异表达。我们建议，SlZFP 2功能作为一个阻遏物微调阿坝的生物合成在果实发育过程中，并提供了一个潜在的有价值的工具，解剖阿坝在果实成熟的作用。
A transcription factor represses abscisic acid biosynthesis and fruit ripening. Abscisic acid (ABA) regulates plant development and adaptation to environmental conditions. Although the ABA biosynthesis pathway in plants has been thoroughly elucidated, how ABA biosynthetic genes are regulated at the molecular level during plant development is less well understood. Here, we show that the tomato (Solanum lycopersicum) zinc finger transcription factor SlZFP2 is involved in the regulation of ABA biosynthesis during fruit development. Overexpression of SlZFP2 resulted in multiple phenotypic changes, including more branches, early flowering, delayed fruit ripening, lighter seeds, and faster seed germination, whereas down-regulation of its expression caused problematic fruit set, accelerated ripening, and inhibited seed germination. SlZFP2 represses ABA biosynthesis during fruit development through direct suppression of the ABA biosynthetic genes NOTABILIS, SITIENS, and FLACCA and the aldehyde oxidase SlAO1. We also show that SlZFP2 regulates fruit ripening through transcriptional suppression of the ripening regulator COLORLESS NON-RIPENING. Using bacterial one-hybrid screening and a selected amplification and binding assay, we identified the (A/T)(G/C)TT motif as the core binding sequence of SlZFP2. Furthermore, by RNA sequencing profiling, we found that 193 genes containing the SlZFP2-binding motifs in their promoters were differentially expressed in 2 d post anthesis fruits between the SlZFP2 RNA interference line and its nontransgenic sibling. We propose that SlZFP2 functions as a repressor to fine-tune ABA biosynthesis during fruit development and provides a potentially valuable tool for dissecting the role of ABA in fruit ripening.