Changes in insulin sensitivity from stress during repetitive sampling in anesthetized rats.
Changes in insulin sensitivity from stress during repetitive sampling in anesthetized rats.
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麻醉大鼠重复采样期间应激引起的胰岛素敏感性变化。
DOI：
10.1152/ajpregu.1992.262.6.r1033
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
R. Rao
中科院分区：
文献类型：
--
作者：
R. Rao
在麻醉大鼠上研究了重复采样对胰岛素敏感性的影响。在葡萄糖钳夹研究期间，累积失血9 ml/kg后，葡萄糖处置从9.3 +/- 0.9（SE）降至6.5 +/- 1.1 mg.kg-1.min-1（P <0.05），肝葡萄糖输出量（HGO）从1.2 +/- 0.8增至2.4 +/- 1.1 mg.kg-1.min-1（P <0.05）。在损失15 ml/kg后，HGO进一步升高至4.7 +/- 1.6 mg.kg-1.min-1（P <0.05）。在相同条件下重复采样期间，尽管同时输注生理盐水，血浆促肾上腺皮质激素（ACTH）仍从68 +/- 11 pg/ml增加至102 +/- 15 pg/ml（P小于0.05），损失8 ml/kg，而血浆胰岛素从39 +/- 7增加到124 +/- 20 mU/l（P小于0.01），损失10 ml/kg。此后，ACTH和胰岛素逐渐升高。血浆皮质酮密切遵循ACTH反应的模式，表明累积失血的应激对肾上腺类固醇的产生有显着影响。ACTH的增加因体积损失减少而延迟，因体积损失增加而加速。得出的结论是，从失血大于7毫升/公斤的压力可能是一个错误的来源，在钳夹实验中的大鼠的葡萄糖周转率和胰岛素敏感性的评价。
The effect of repetitive sampling on insulin sensitivity was studied in anesthetized rats. During glucose clamp studies, glucose disposal decreased from 9.3 +/- 0.9 (SE) to 6.5 +/- 1.1 mg.kg-1.min-1 (P less than 0.05), and hepatic glucose output (HGO) increased from 1.2 +/- 0.8 to 2.4 +/- 1.1 mg.kg-1.min-1 (P less than 0.05) after a cumulative blood loss of 9 ml/kg. After a loss of 15 ml/kg, HGO rose further to 4.7 +/- 1.6 mg.kg-1.min-1 (P less than 0.05). During repetitive sampling under identical conditions, plasma adrenocorticotropic hormone (ACTH) increased, despite simultaneous saline infusion, from 68 +/- 11 to 102 +/- 15 pg/ml (P less than 0.05) with a loss of 8 ml/kg, while plasma insulin increased from 39 +/- 7 to 124 +/- 20 mU/l (P less than 0.01) with a loss of 10 ml/kg. Thereafter, ACTH and insulin rose progressively. Plasma corticosterone closely followed the pattern of the ACTH response, indicating that the stress of cumulative blood loss had a significant effect on adrenal steroid production. Increases in ACTH were retarded by reduced volume loss and accelerated by increased loss. It is concluded that stress from blood loss greater than 7 ml/kg may be a source of error in the evaluation of glucose turnover and insulin sensitivity during clamp experiments in rats.