In the non-insect-transmissible line of onion yellows phytoplasma (OY-NIM), the plasmid-encoded transmembrane protein ORF3 lacks the major promoter region
In the non-insect-transmissible line of onion yellows phytoplasma (OY-NIM), the plasmid-encoded transmembrane protein ORF3 lacks the major promoter region
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DOI：
10.1099/mic.0.027409-0
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发表时间：
2009-06-01
期刊：
影响因子：
2.8
通讯作者：
Namba, Shigetou
中科院分区：
文献类型：
--
作者：
Ishii, Yoshiko;Kakizawa, Shigeyuki;Namba, Shigetou
洋葱黄化菌株（OY）是一株由粗中柱叶蝉（Macrosteles striifrons leafhoppers）传播的植物病原细菌。OY-M含有两种类型的质粒（EcOYM和pOYM），每种质粒都具有编码推定的跨膜蛋白ORF 3的基因。该植原体的非昆虫传播系（OY-NIM）具有相应的质粒（EcOYNIM和pOYNIM），但pOYNIM缺乏orf 3。在这里，我们表明，在OY-M，orf 3转录从两个假定的启动子和EcOYNIM，启动子序列之一是突变和其他删除。我们还表明，ORF 3在OY-NIM感染的植物中不表达的免疫组化分析。此外，ORF 3蛋白似乎优先在OY-M感染的昆虫而不是在植物中表达。推测ORF 3可能在OY与昆虫宿主的相互作用中起一定作用。
'Candidatus Phytoplasma asteris', onion yellows strain (OY), a mildly pathogenic line (OY-M), is a phytopathogenic bacterium transmitted by Macrosteles striifrons leafhoppers. OY-M contains two types of plasmids (EcOYM and pOYM), each of which possesses a gene encoding the putative transmembrane protein, ORF3. A non-insect-transmissible line of this phytoplasma (OY-NIM) has the corresponding plasmids (EcOYNIM and pOYNIM), but pOYNIM lacks orf3. Here we show that in OY-M, orf3 is transcribed from two putative promoters and that on EcOYNIM, one of the promoter sequences is mutated and the other deleted. We also show by immunohistochemical analysis that ORF3 is not expressed in OY-NIM-infected plants. Moreover, ORF3 protein seems to be preferentially expressed in OY-M-infected insects rather than in plants. We speculate that ORF3 may play a role in the interactions of OY with its insect host.