Surfaces having dual fibrinolytic and protein resistant properties by immobilization of lysine on polyurethane through a PEG spacer
Surfaces having dual fibrinolytic and protein resistant properties by immobilization of lysine on polyurethane through a PEG spacer
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通过 PEG 间隔基将赖氨酸固定在聚氨酯上，使表面具有双重纤溶和抗蛋白质特性
DOI：
10.1002/jbm.a.32152
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发表时间：
2009-09-01
影响因子：
4.9
通讯作者：
Brash, John L.
中科院分区：
文献类型：
--
作者：
Chen, Hong;Zhang, Yanxia;Brash, John L.
这项工作的目标是开发一种既能抵抗非特异性蛋白质吸附又能溶解血栓的血液接触表面。用聚乙二醇(PEG)对聚氨酯(PU)表面进行化学修饰；赖氨酸被用来产生与纤溶酶原结合的潜在纤溶表面。该制备过程包括用二羟基聚乙二醇改性PU表面，未反应的远端羟基与N，N‘-二琥珀酰亚胺碳酸酯(DSC)反应生成PU-PEG-NHS表面，然后通过α-氨基与NHS反应偶联α-氨基保护的赖氨酸(H-Lys(t-BOC)-OH)，以及去保护。其结果是赖氨酸衍生的表面，其中赖氨酸的epsilon-氨基自由参与结合纤溶酶原和组织纤溶酶原激活物(t-PA)。用X射线光电子能谱(XPS)和接触角测量对其表面进行了表征。蛋白质吸附实验表明，非特异性蛋白质在这些表面上的吸附大大减少，并从血浆中吸附了大量的纤溶酶原。在与血浆孵育和t-PA处理后，表面能够溶解在其周围形成的新生血浆凝块。(C)2008 Wiley期刊，Inc.《生物医学杂志》90A：940-946,2009
The objective of this work is to develop a blood contacting surface that possesses both resistance to nonspecific protein adsorption and clot lysing properties. Chemical modification of a polyurethane (PU) surface with poly(ethylene glycol) (PEG); and lysine was used to create a plasminogen-binding potentially fibrinolytic surface. The preparation involves modification of the PU surface with dihydroxy PEG, reaction of the unreacted distal OH with N,N'-disuccinimidyl carbonate (DSC) to produce a PU-PEG-NHS surface, followed by conjugation of alpha-amino-protected lysine (H-Lys(t-BOC)-OH) by reaction of the alpha-amino group with the NHS and deprotection. The result is a lysine-derivatized surface in which the epsilon-amino groups of the lysine are free to participate in binding plasminogen and tissue plasminogen activator (t-PA). Surfaces were characterized by X-ray photoelectron spectroscopy (XPS) and contact angle measurements. Protein adsorption experiments showed that nonspecific protein adsorption was greatly reduced on these surfaces and that they adsorbed significant quantities of plasminogen from plasma. After incubation with plasma and treatment with t-PA the surfaces were able to dissolve nascent plasma clots formed around them. (C) 2008 Wiley Periodicals, Inc. J Biomed Mater Res 90A: 940-946, 2009