Electrochemical assay to detect influenza viruses and measure drug susceptibility.
Electrochemical assay to detect influenza viruses and measure drug susceptibility.
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DOI：
10.1002/anie.201412164
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发表时间：
2015-05-11
期刊：
影响因子：
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通讯作者：
Iyer SS
中科院分区：
文献类型：
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作者：
Zhang X;Dhawane AN;Sweeney J;He Y;Vasireddi M;Iyer SS
我们设计了一种电化学检测方法来快速诊断流感病毒。将含葡萄糖的底物暴露于流感病毒或其酶，神经氨酸酶（NA）释放葡萄糖，用电流法检测。我们用两种方法检测释放葡萄糖。首先，我们使用标准血糖仪检测两种病毒NAs和三种流感毒株。我们还利用该方法证明了两种抗病毒药物扎那米韦和奥司他韦的药物敏感性。其次，我们使用一次性试纸在1小时内检测了19种H1N1和H3N2流感病毒株。第一代测定法的检测限和范围分别为102和102 - 108斑块形成单位（pfu）。现有的、无处不在的、用户友好的血糖仪可以重新用于检测流感病毒。
We have designed an electrochemical assay to rapidly diagnose influenza viruses. Exposure of a glucose bearing substrate to influenza viruses or its enzyme, Neuraminidase (NA) releases glucose, which was detected amperometrically. We used two methods to detect released glucose. First, we used a standard glucose blood meter to detect two viral NAs and three influenza strains. We also demonstrated drug susceptibility of two antivirals, Zanamivir and Oseltamivir, using the assay. Second, we used disposable test strips to detect nineteen H1N1 and H3N2 influenza strains using this assay in 1 hour. The limit and range of detection of this first generation assay is 102 and 102–108 plaque forming units (pfu), respectively. Existing, ubiquitous, user friendly glucose meters can be repurposed to detect influenza viruses.