Visualization of the moment of mouse sperm-egg fusion and dynamic localization of IZUMO1
Visualization of the moment of mouse sperm-egg fusion and dynamic localization of IZUMO1
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DOI：
10.1242/jcs.100867
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发表时间：
2012-11-01
影响因子：
4
通讯作者：
Okabe, Masaru
中科院分区：
文献类型：
--
作者：
Satouh, Yuhkoh;Inoue, Naokazu;Okabe, Masaru
基因破坏实验已经证明顶体蛋白IZUMO 1是小鼠精卵融合所必需的。然而，尽管其预测的功能，它不是在射精精子的表面上表达。在这里，我们报告的动态扩散IZUMO 1从顶体膜的精子表面的顶体反应时，可视化使用荧光蛋白标签。顶体反应后，IZUMO 1有定位于精子表面赤道段的趋势。这个区域被认为是开始与卵膜融合的区域。精卵融合的瞬间和融合过程中蛋白质的动态运动也被实时成像。阐明了IZUMO 1在受精过程中的易位，并推测了哺乳动物受精的基本机制。
Gene disruption experiments have proven that the acrosomal protein IZUMO1 is essential for sperm-egg fusion in the mouse. However, despite its predicted function, it is not expressed on the surface of ejaculated spermatozoa. Here, we report the dynamics of diffusion of IZUMO1 from the acrosomal membrane to the sperm surface at the time of the acrosome reaction, visualized using a fluorescent protein tag. IZUMO1 showed a tendency to localize in the equatorial segment of the sperm surface after the acrosome reaction. This region is considered to initiate fusion with the oolemma. The moment of sperm-egg fusion and the dynamic movements of proteins during fusion were also imaged live. Translocation of IZUMO1 during the fertilization process was clarified, and a fundamental mechanism in mammalian fertilization is postulated.