Genetic Manipulation of Stenotrophomonas maltophilia.
Genetic Manipulation of Stenotrophomonas maltophilia.
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DOI：
10.1002/9780471729259.mc06f02s37
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发表时间：
2015-05-01
影响因子：
--
通讯作者：
Fisher N
中科院分区：
文献类型：
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作者：
Welker E;Domfeh Y;Tyagi D;Sinha S;Fisher N
嗜麦芽窄食单胞菌是一种革兰氏阴性、需氧、能动、环境细菌，是一种重要的医院内致病菌，在重症患者中具有很高的归因死亡率。嗜麦芽窄食单胞菌是囊性纤维化(CF)患者特别关注的问题，因为它已被证明定植于呼吸道上皮细胞并建立慢性感染。在这里，我们介绍了几种用于该细菌的遗传操作的分子技术，包括DNA提取、RNA提取、从大肠杆菌中连接质粒和等位基因交换。
Stenotrophomonas maltophilia is a Gram-negative, aerobic, motile, environmental bacterium that is emerging as an important nosocomial pathogen with high rates of attributable mortality in severely ill patients. S. maltophilia is of particular concern to patients suffering from cystic fibrosis (CF) as it has been shown to colonize airway epithelial and establish a chronic infection. Here we describe several molecular techniques for the genetic manipulation of this bacterium, including DNA extraction, RNA extraction, conjugation of plasmids from E. coli and allelic exchange.