Interaction between the small GTPase Ran/Gsp1p and Ntf2p is required for nuclear transport
Interaction between the small GTPase Ran/Gsp1p and Ntf2p is required for nuclear transport
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DOI：
10.1128/mcb.17.7.3755
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发表时间：
1997-07-01
影响因子：
5.3
通讯作者：
Silver, PA
中科院分区：
文献类型：
--
作者：
Wong, DH;Corbett, AH;Silver, PA
蛋白质和RNA在核膜上的双向移动需要RAN，一个类似RAS的GTP酶。对酿酒酵母进行遗传筛选，以分离编码RAN同源基因GSP1的条件等位基因，分离出两个温度敏感等位基因gsp1-1和gsp1-2。这两个等位基因的突变定位于RAS家族不同成员之间结构保守的区域，每个突变株都表现出不同的核运输缺陷，生化和遗传学实验表明，蛋白质输入所需的因子Ntf2p与突变的GSP1基因产物之间的相互作用减少，NTF2的过表达可以抑制gsp1-1和gsp1-2的温度敏感表型，部分挽救核运输缺陷。然而，过度表达与Gsp1p结合减少的NTF2突变等位基因并不能挽救gsp1-1和gsp1-2的温度敏感性。综上所述，这些数据表明Gsp1p和Ntf2p之间的相互作用对核运输至关重要。
Bidirectional movement of proteins and RNAs across the nuclear envelope requires Ran, a Ras-like GTPase, A genetic screen of the yeast Saccharomyces cerevisiae was performed to isolate conditional alleles of GSP1, a gene that encodes a homolog of Ran, Two temperature-sensitive alleles, gsp1-1 and gsp1-2, were isolated. The mutations in these two alleles map to regions that are structurally conserved between different members of the Ras family, Each mutant strain exhibits various nuclear transport defects, Both biochemical and genetic experiments indicate a decreased interaction between Ntf2p, a factor which is required for protein import, and the mutant GSP1 gene products, Overexpression of NTF2 can suppress the temperature sensitive phenotype of gsp1-1 and gsp1-2 and partially rescue nuclear transport defects. However, overexpression of a mutant allele of NTF2 with decreased binding to Gsp1p cannot rescue the temperature sensitivity of gsp1-1 and gsp1-2. Taken together, these data demonstrate that the interaction between Gsp1p and Ntf2p is critical for nuclear transport.