Chromosomal rearrangements underlying karyotype differences between Chinese pangolin (Manis pentadactyla) and Malayan pangolin (Manis javanica) revealed by chromosome painting
Chromosomal rearrangements underlying karyotype differences between Chinese pangolin (Manis pentadactyla) and Malayan pangolin (Manis javanica) revealed by chromosome painting
复制标题
染色体涂色揭示中华穿山甲（Manis pentadactyla）和马来穿山甲（Manis javanica）核型差异背后的染色体重排
DOI：
10.1007/s10577-009-9027-0
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发表时间：
2009-04-01
影响因子：
2.6
通讯作者：
Yang, Fengtang
中科院分区：
文献类型：
--
作者：
Nie, Wenhui;Wang, Jinhuan;Yang, Fengtang
中国穿山甲是石仙桃目的代表性物种，由于其在系统发育上的重要性而被列入哺乳动物全基因组测序计划。以往的研究表明，M.五趾龙可以从2n = 36变化到42。为了进一步研究M.为了阐明石仙桃属植物核型多样性的染色体机制，我们对2n = 40 M的石仙桃属植物染色体进行了流式分选。pentadactyla的，并产生了一组染色体特异性探针，通过DOP-PCR扩增流式分选染色体。并绘制了M. pentadactyla与马来穿山甲（Manis javanica，2n = 38）之间以及人与M. pentadactyla首次通过染色体涂绘建立。我们的研究结果表明，7罗伯逊重排，以及相当大的变化，异染色质的数量和核仁组成区（NORs）的数目区分2n = 38 M的核型。javanica，2n = 40M。五趾类。此外，我们还证实了M. javanicaY染色体有一个NOR。在M. javanica和M. pentadactylaHSA 1 q/11、2 p/5、2 q/10 q、4p+q/20、5/13、6/19 p和8 q/10 p等7个共同的关联可能构成石仙桃门特有的信号重排。
The Chinese pangolin (Manis pentadactyla), a representative species of the order Pholidota, has been enlisted in the mammalian whole-genome sequencing project mainly because of its phylogenetic importance. Previous studies showed that the diploid number ofM. pentadactylacould vary from 2n = 36 to 42. To further characterize the genome organization ofM. pentadactylaand to elucidate chromosomal mechanism underlying the karyotype diversity of Pholidota, we flow-sorted the chromosomes of 2n = 40M. pentadactyla, and generated a set of chromosome-specific probes by DOP-PCR amplification of flow-sorted chromosomes. A comparative chromosome map betweenM. pentadactylaand the Malayan pangolin (Manis javanica, 2n = 38), as well as between human andM. pentadactyla, was established by chromosome painting for the first time. Our results demonstrate that seven Robertsonian rearrangements, together with considerable variations in the quantity of heterochromatin and in the number of nucleolar organizer regions (NORs) differentiate the karyotypes of 2n = 38M. javanicaand 2n = 40M. pentadactyla. Moreover, we confirm that theM. javanicaY chromosome bears one NOR. Comparison of human homologous segment associations found in the genomes ofM. javanicaandM. pentadactylarevealed seven shared associations (HSA 1q/11, 2p/5, 2q/10q, 4p+q/20, 5/13, 6/19p and 8q/10p) that could constitute the potential Pholidota-specific signature rearrangements.