Comparative proteomic analysis of a membrane-enriched fraction from flag leaves reveals responses to chemical hybridization agent SQ-1 in wheat.
Comparative proteomic analysis of a membrane-enriched fraction from flag leaves reveals responses to chemical hybridization agent SQ-1 in wheat.
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剑叶膜富集部分的比较蛋白质组分析揭示了小麦对化学杂交剂 SQ-1 的反应
DOI：
10.3389/fpls.2015.00669
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发表时间：
2015
影响因子：
5.6
通讯作者：
Ma S
中科院分区：
文献类型：
--
作者：
Song Q;Wang S;Zhang G;Li Y;Li Z;Guo J;Niu N;Wang J;Ma S
利用化学杂交剂SQ-1(CHA-SQ-1)诱导小麦雄性可育系是利用杂种优势的有效途径，但雄性可育的分子基础尚不清楚。小麦旗叶是CHA-SQ-1的初始受体，其膜结构在响应CHA-SQ-1胁迫过程中起着至关重要的作用。为了研究小麦旗叶对CHA-SQ-1胁迫的响应，我们比较了它们在没有CHA-SQ-1和存在CHA-SQ-1的情况下的定量蛋白质组图谱。我们的结果表明，在CHA-SQ-1处理过程中，小麦旗叶遭受了氧化胁迫。在CHA-SQ-1处理后10h内，叶片O2-、H2O2和丙二醛含量显著增加，而超氧化物歧化酶、过氧化氢酶和愈创木酚过氧化物酶等主要抗氧化酶活性显著降低。膜富集组分的蛋白质组谱显示，参与多种生物过程的一组膜蛋白的丰度发生了变化。这些可变蛋白主要影响光合作用、ATP合成的蛋白机制，并参与逆境反应。这些结果解释了膜蛋白质组与花药败育的关系，并为CHA在杂交育种中的实际应用提供了理论依据。
The induction of wheat male fertile lines by using the chemical hybridizing agent SQ-1 (CHA-SQ-1) is an effective approach in the utilization of heterosis; however, the molecular basis of male fertility remains unknown. Wheat flag leaves are the initial receptors of CHA-SQ-1 and their membrane structure plays a vital role in response to CHA-SQ-1 stress. To investigate the response of wheat flag leaves to CHA-SQ-1 stress, we compared their quantitative proteomic profiles in the absence and presence of CHA-SQ-1. Our results indicated that wheat flag leaves suffered oxidative stress during CHA-SQ-1 treatments. Leaf O2-, H2O2, and malonaldehyde levels were significantly increased within 10 h after CHA-SQ-1 treatment, while the activities of major antioxidant enzymes such as superoxide dismutase, catalase, and guaiacol peroxidase were significantly reduced. Proteome profiles of membrane-enriched fraction showed a change in the abundance of a battery of membrane proteins involved in multiple biological processes. These variable proteins mainly impaired photosynthesis, ATP synthesis protein mechanisms and were involved in the response to stress. These results provide an explanation of the relationships between membrane proteomes and anther abortion and the practical application of CHA for hybrid breeding.