Handheld instrument for wound-conformal delivery of skin precursor sheets improves healing in full-thickness burns
Handheld instrument for wound-conformal delivery of skin precursor sheets improves healing in full-thickness burns
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DOI：
10.1088/1758-5090/ab6413
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发表时间：
2020-04-01
期刊：
影响因子：
9
通讯作者：
Gunther, Axel
中科院分区：
文献类型：
--
作者：
Cheng, Richard Y.;Eylert, Gertraud;Gunther, Axel
目前对严重大面积烧伤患者的护理标准包括自体皮肤移植或脱细胞真皮替代物。虽然加速伤口愈合的新兴选择涉及用同种异体或自体细胞治疗，但将细胞递送到临床相关的伤口拓扑结构、方向和大小仍然是一个挑战。在这里，我们报告的一步原位形成的含细胞的生物材料片使用手持仪器，适应地形的伤口。在维持细胞活力和增殖的方法中，我们证明了向相对于水平方向倾斜高达45度的表面的共形递送。在猪全层烧伤的临床前模型中，我们将含有间充质干细胞/基质细胞的纤维蛋白片直接递送到伤口床，改善了上皮再生、真皮细胞再生和新血管形成，表明该装置可以引入临床环境中，改善真皮和表皮再生。
The current standard of care for patients with severe large-area burns consists of autologous skin grafting or acellular dermal substitutes. While emerging options to accelerate wound healing involve treatment with allogeneic or autologous cells, delivering cells to clinically relevant wound topologies, orientations, and sizes remains a challenge. Here, we report the one-step in situ formation of cell-containing biomaterial sheets using a handheld instrument that accommodates the topography of the wound. In an approach that maintained cell viability and proliferation, we demonstrated conformal delivery to surfaces that were inclined up to 45 degrees with respect to the horizontal. In porcine pre-clinical models of full-thickness burn, we delivered mesenchymal stem/stromal cell-containing fibrin sheets directly to the wound bed, improving re-epithelialization, dermal cell repopulation, and neovascularization, indicating that this device could be introduced in a clinical setting improving dermal and epidermal regeneration.