ATTENUATION OF ISCHEMIC RENAL INJURY WITH FRUCTOSE 1,6-DIPHOSPHATE
ATTENUATION OF ISCHEMIC RENAL INJURY WITH FRUCTOSE 1,6-DIPHOSPHATE
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DOI：
10.1016/0022-4804(89)90111-x
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发表时间：
1989-09-01
影响因子：
2.2
通讯作者：
MARKOV, AK
中科院分区：
文献类型：
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作者：
DIDLAKE, R;KIRCHNER, KA;MARKOV, AK
果糖1，6-二磷酸（FDP）已被证明可以通过增强无氧碳水化合物的利用和抑制中性粒细胞产生氧自由基来减轻与缺血和休克相关的组织损伤。以前，我们曾报道，FDP预防缺血性肾功能衰竭，如果在缺血性损伤之前给药。本研究旨在确定这种药物是否可以防止肾损伤时，在缺血后再灌注期间管理。大鼠进行30分钟的双侧肾动脉闭塞和FDP（350毫克/公斤体重）开始后10分钟释放肾动脉夹输注。对照组大鼠接受等体积的葡萄糖/盐水溶液。第三组大鼠进行假手术。损伤后24小时，FDP治疗组大鼠的BUN、肌酐和钠排泄分数值低于对照组大鼠（分别为P <0.001、P <0.005和P <0.001），与假手术组大鼠的观察值无差异。FDP处理的大鼠中菊糖清除率（P <0.001）大于对照大鼠（665 ± 0.001）。38 μ l/min/g肾重量）。FDP治疗组的肾组织学也保存得更好。这些数据表明，急性缺血性损伤开始后输注FDP提供了显著的，但不完全的，功能和组织学保护免受肾损伤。
Fructose 1,6-diphosphate (FDP) has been shown to attenuate tissue injury associated with ischemia and shock by enhancing the anaerobic carbohydrate utilization and by inhibiting oxygen-free-radical generation by the neutrophils. Previously, we have reported that FDP prevents ischemic renal failure if administered prior to the ischemic insult. The present study was designed to determine whether this agent could prevent renal damage when administered during the postischemic reperfusion period. Rats were subjected to 30 min of bilateral renal artery occlusion and infused with FDP (350 mg/kg body wt) beginning 10 min after release of the renal artery clamps. Control rats received an equal volume of glucose/saline solution. A third group of rats were sham operated. Twenty-four hours after injury, BUN, creatinine, and fractional sodium excretion values were less in FDP-treated rats than in control rats (P < 0.001, P < 0.005, and P < 0.001, respectively) and not different from values observed in sham-operated rats. Inulin clearance was greater (P < 0.001) in FDP-treated rats than in control rats (665 .+-. 38 .mu.l/min/g kidney wt). Renal histology was also better preserved in the FDP-treated group. These data suggest that FDP infused after the initiation of an acute ischemic insult provides significant, but not complete, functional and histologic protection from renal damage.