Transcriptomic Analysis of the Molecular Mechanisms Underlying the Antibacterial Activity of IONPs@pDA-Nisin Composites toward Alicyclobacillus acidoterrestris
Transcriptomic Analysis of the Molecular Mechanisms Underlying the Antibacterial Activity of IONPs@pDA-Nisin Composites toward Alicyclobacillus acidoterrestris
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IONPs@pDA-乳链菌肽复合物对酸土脂环酸芽孢杆菌抗菌活性分子机制的转录组分析
DOI：
10.1021/acsami.9b02990
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发表时间：
2019-06-19
影响因子：
9.5
通讯作者：
Yue, Tianli
中科院分区：
文献类型：
--
作者：
Song, Zihan;Niu, Chen;Yue, Tianli
通过制备耐热耐pH的乳酸链球菌素（nisin）负载氧化铁纳米粒子/聚多巴胺（IONP @pDA）复合物，开发了一种简单、无耐药性的抗菌方法。复合材料具有晶体结构和25 +/- 3 nm的直径，具有43.7995 emu g（-1）的饱和磁化强度（M-s）。傅立叶变换红外光谱和X射线光电子能谱分析表明，乳酸链球菌素成功地结合到了IONP @pDA纳米颗粒上。新合成的材料在减少Alicyclobacillus acidoterrestris方面表现出良好的性能，Alicyclobacillus acidoterrestris是一种常见的食品腐败细菌，代表了食品工业的一个重大问题。A的处理。如通过活/死染色和扫描电子显微镜和透射电子显微镜分析所观察到的，具有复合物的酸土芽孢杆菌细胞导致膜损伤。此外，该复合材料表现出高效的抗菌活性，对细胞在只有5分钟。转录组测序的文化RNA池暴露后的复合材料导致共334个差异表达的基因，主要与转录调控，能量代谢，膜转运蛋白，膜和细胞壁合成，和细胞运动。因此，这些结果表明，由靶细胞上聚集的复合物引起的转录调控的变化导致稳态的重大变化，其表现为能量代谢降低、膜中的孔形成和抑制的细胞壁合成。同时，细胞运动和孢子形成活动都受到抑制，最终，胞内物质流出渗漏细胞。提出的生物防治方法代表了一种新的手段来控制微生物，而不会诱导耐药性。此外，这些结果提供了新的见解的分子机制的复合材料对微生物的抗菌活性。
A simple and no-drug resistance antibacterial method was developed by the synthesis of heat-stable and pH-tolerant nisin-loaded iron oxide nanoparticles polydopamine (IONPs@pDA) composites. The composites had a crystal structure and diameters of 25 +/- 3 nm, with a saturation magnetization (M-s) of 43.7995 emu g(-1). Nisin was successfully conjugated onto the IONPs@pDA nanoparticles, as evinced by Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy analyses. The novel synthesized material showed good performance in reducing Alicyclobacillus acidoterrestris, a common food spoilage bacterium that represents a significant problem for the food industry. Treatment of A. acidoterrestris cells with composites resulted in membrane damage, as observed by live/dead staining and scanning electron microscopy and transmission electron microscopy analyses. Further, the composites exhibited highly efficient antibacterial activity against cells in only 5 min. Transcriptomic sequencing of culture RNA pools after exposure to composites resulted in a total of 334 differentially expressed genes that were primarily associated with transcriptional regulation, energy metabolism, membrane transporters, membrane and cell wall syntheses, and cell motility. Thus, these results suggested that changes in transcriptional regulation caused by aggregated composites on target cells led to major changes in homeostasis that manifested by decreased energy metabolism, pore formation in the membrane, and repressed cell wall synthesis. Concomitantly, cell motility and sporulation activities were both repressed, and finally, intracellular substances flowed out of leaky cells. The proposed biocontrol method represents a novel means to control microorganisms without inducing drug resistance. Further, these results provide novel insights into the molecular mechanisms underlying the antibacterial activity of composites against microorganisms.