Bidirectional synaptic plasticity in the dentate gyrus of the awake freely behaving mouse.
Bidirectional synaptic plasticity in the dentate gyrus of the awake freely behaving mouse.
复制标题
清醒自由行为小鼠齿状回的双向突触可塑性。
DOI：
10.1016/j.jneumeth.2007.08.001
复制
发表时间：
2008
影响因子：
3
通讯作者：
Blaise,JHarry
中科院分区：
文献类型：
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作者：
Koranda,JessicaL;Masino,SusanA;Blaise,JHarry
由于现在有许多相关的遗传突变体，小鼠体内突触可塑性有很大的兴趣。然而，体内模型的使用仍然有限。迄今为止，长时程增强（LTP）的研究很少，长时程抑制（LTD）尚未在小鼠体内的特点。在这里，我们描述的协议和改进的方法，我们开发的记录海马突触可塑性可靠的清醒自由行为的小鼠的齿状回。在记录前7天，我们植入了封装在轻质、低切迹头部载物台组件内的微电极。在记录当天，我们在清醒的自由行为动物中诱导LTP或LTD，并监测群体尖峰幅度的后续变化至少24小时。使用此协议，我们达到了80%的成功诱导和维持无论是LTP或LTD。记录从一个长期的植入物，使用这种改进的方法是最适合揭示自然发生的大脑活动，并避免了急性效应的局部电极插入和漂移与麻醉相关的神经元兴奋性。最终，一个可靠的自由行为的双向突触可塑性的小鼠模型是非常宝贵的疾病状态的遗传模型的充分表征和操纵的机制，涉及学习和记忆。
There is significant interest in in vivo synaptic plasticity in mice due to the many relevant genetic mutants now available. Nevertheless, use of in vivo models remains limited. To date long-term potentiation (LTP) has been studied infrequently, and long-term depression (LTD) has not been characterized in the mouse in vivo. Herein we describe protocols and improved methodologies we developed to record hippocampal synaptic plasticity reliably from the dentate gyrus of the awake freely behaving mouse. Seven days prior to recording, we implanted microelectrodes encapsulated within a lightweight, low profile head stage assembly. On the day of recording, we induced either LTP or LTD in the awake freely behaving animal, and monitored subsequent changes in population spike amplitude for at least 24h. Using this protocol we attained 80% success in inducing and maintaining either LTP or LTD. Recording from a chronic implant using this improved methodology is best suited to reveal naturally occurring brain activity and avoids both acute effects of local electrode insertion and drifts in neuronal excitability associated with anesthesia. Ultimately a reliable freely behaving mouse model of bi-directional synaptic plasticity is invaluable for full characterization of genetic models of disease states and manipulations of the mechanisms implicated in learning and memory.