Induction of oxidative stress in the tissues of rats after chronic exposure to TCDD, 2,3,4,7,8-pentachlorodibenzofuran, and 3,3′,4,4′,5-pentachlorobiphenyl
Induction of oxidative stress in the tissues of rats after chronic exposure to TCDD, 2,3,4,7,8-pentachlorodibenzofuran, and 3,3′,4,4′,5-pentachlorobiphenyl
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DOI：
10.1080/00984100290071054
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发表时间：
2002-06-28
影响因子：
2.6
通讯作者：
Stohs, SJ
中科院分区：
文献类型：
--
作者：
Hassoun, EA;Wang, H;Stohs, SJ
研究了2，3，7，8-四氯二苯并-对二恶英(TCDD)、2，3，4，7，8-五氯二苯并呋喃(PeCDF)、3，3，4，4，5-五氯联苯(PB126)及其混合物(毒性当量，TEQs)对大鼠肝脏和脑组织氧化应激的影响。TCDD、PeCDF、PCB126和TEQ按与其毒性当量因子(TEF)相对应的剂量每天给予各组大鼠，并在暴露结束时测定肝和脑组织中氧化应激的生物标志物，包括超氧阴离子产生、脂质过氧化和DNA单链断裂(SSB)。这三种化学物质在超氧阴离子、脂质过氧化和DNA SSB的产生方面引起了类似的剂量依赖性增加，在一定的剂量范围内保持稳定，然后在较高的剂量水平下继而增加。TEQ也产生了类似的影响；然而，氧化应激生物标志物的剂量依赖性增加是持续的，从未达到平台期水平。除了PCB126外，统计分析显示，与肝组织相比，脑组织中超氧阴离子和脂质过氧化的产生更多，而对其他异物或TEQs的反应，两种组织之间没有显著差异。当将TEQ引起的影响与单独化学物质引起的影响进行比较时，也显示出不显著的差异。
The abilities of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), 2,3,4,7,8-pentachlorodibenzofutan (PeCDF), 3,3,4,4,.5-pentachlorobiphenyl (PB126), and mixtures of these xenobiotics (toxic equivalents, TEQs) to induce oxidative stress in hepatic and brain tissues of rats have been investigated after chronic (30 wk) exposure to these congeners. TCDD, PeCDF, PCB126, and TEQs were administered daily to groups of rats at doses that corresponded to their toxic equivalency factors (TEFs), and the biomarkers of oxidative stress, including the production of superoxide anion, lipid peroxidation, and DNA single-strand breaks (SSBs), were determined in hepatic and brain tissues at the end of the exposure period. The three chemicals caused similar dose-dependent increases in the production Of Superoxide anion, lipid peroxidation, and DNA SSBs, which plateaued at certain dose ranges, followed by secondary increases at the higher dose levels. Similar effects were also produced by the TEQs; however, the dose-dependent increases in the biomarkers of oxidative stress were continuous and never achieved plateau levels. Except for PCB126, where statistical analyses revealed greater productions of superoxide anion and lipid peroxidation in brain tissues as compared with hepatic tissues, no significant differences were revealed between the two tissues in response to the other xenobiotics or the TEQs. Nonsignificant differences were also revealed when comparing the effects induced by the TEQs with those induced by the individual chemicals.