Different localization in rat brain of the novel cytosolic ketone body-utilizing enzyme, acetoacetyl-CoA synthetase, as compared to succinyl-CoA:3-oxoacid CoA-transferase
Different localization in rat brain of the novel cytosolic ketone body-utilizing enzyme, acetoacetyl-CoA synthetase, as compared to succinyl-CoA:3-oxoacid CoA-transferase
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DOI：
10.1016/j.bbaexp.2005.05.006
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发表时间：
2005-07-10
期刊：
影响因子：
--
通讯作者：
Fukui, T
中科院分区：
文献类型：
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作者：
Ohnuki, M;Takahashi, N;Fukui, T
在生脂组织胞浆中，酮体被乙酰乙酰-辅酶A合成酶(AACS)激活，并被结合到胆固醇和脂肪酸中。为了研究AACs在脑内的生理作用，我们用标记的探针，用原位杂交的方法检测了AACs在大鼠脑内的表达。在中脑、脑桥/延髓、大脑皮层、海马区和小脑均有高标记，其定位谱与线粒体酮体活化酶琥珀酰辅酶A：3-氧酸辅酶A转移酶(SCOT)不同。此外，AACS mRNA在小脑中的表达主要局限于神经胶质细胞，而在大脑皮层中仅限于神经细胞。链脲佐菌素处理可显著降低AACSm RNA表达水平，但对Scot m RNA水平无明显影响。这些结果表明，AAC的生理作用不同于SCOT，并且根据其在脑中的定位而不同。(C)2005 Elsevier B.V.保留所有权利。
In lipogenic tissue cytosol, ketone bodies are known to be activated by acetoacetyl-CoA synthetase (AACS) and incorporated into cholesterol and fatty acids. In order to investigate the physiological role of AACS in the brain, we examined the localization of AACS mRNA in rat brain by in situ hybridization using a labeled probe. High labeling was observed in the midbrain, pons/medulla, cerebral cortex, hippocampus and cerebellum, and the localization profile of AACS mRNA was different from that of succinyl-CoA:3-oxoacid CoA-transferase (SCOT), a mitochondrial ketone body-activating enzyme. In addition, the expression of AACS mRNA in the cerebellum was restricted primarily to glial cells, while in the cerebral cortex, it was restricted to neuronal cells. Streptozotocin treatment caused remarkable decreases in AACS mRNA levels in all regions where expression was observed, but changes in SCOT mRNA levels were not observed. These results suggest that the physiological role of AACS is different from that of SCOT and varies depending upon its localization in the brain. (c) 2005 Elsevier B.V. All rights reserved.