Assessment of transferrin recycling by Triplet Lifetime Imaging in living cells.
Assessment of transferrin recycling by Triplet Lifetime Imaging in living cells.
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DOI：
10.1364/boe.3.002526
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发表时间：
2012-10-01
影响因子：
3.4
通讯作者：
Lasser T
中科院分区：
文献类型：
--
作者：
Geissbuehler M;Kadlecova Z;Klok HA;Lasser T
提出了一种测量荧光分子三重态寿命的光学方法。这是每个荧光团特有的特征。基于两种荧光物质（自发荧光与标记）的三重态寿命的差异，这种新方法测量了跨膜受体和相关荧光标记配体在活细胞中再循环期间的相对量。类似于基于荧光寿命的方法，我们的方法对光漂白几乎不敏感。一个简单的理论解混两个已知的三重寿命提出沿着与验证的方法，通过测量转铁蛋白回收的模型系统的基础上，中国仓鼠卵巢细胞（CHO）。转铁蛋白是将Fe 3+运送到细胞的载体。
An optical method is presented that allows the measurement of the triplet lifetime of a fluorescent molecule. This is a characteristic specific to each fluorophore. Based on differences in triplet lifetimes of two fluorescent species (autofluorescence versus label), this novel approach measures relative quantities of a transmembrane receptor and associated fluorescently labeled ligand during its recycling in living cells. Similarly to fluorescence-lifetime based methods, our approach is almost insensitive to photobleaching. A simple theory for unmixing two known triplet lifetimes is presented along with validation of the method by measurements of transferrin recycling in a model system based on chinese hamster ovarian cells (CHO). Transferrin is the delivery carrier for Fe3+ to the cell.