Characterization of inflammatory mediator release from purified human lung mast cells.
Characterization of inflammatory mediator release from purified human lung mast cells.
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纯化的人肺肥大细胞释放炎症介质的表征。
DOI：
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发表时间：
1986
期刊：
影响因子：
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通讯作者：
L. Lichtenstein
中科院分区：
文献类型：
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作者：
Robert P. Schleimer;D. MacGlashan;Stephen P. Peters;R. N. Pinckard;N. Adkinson;L. Lichtenstein
从纯化的人肺肥大细胞中释放炎症介质（组胺、PGD2、TxB2和LTC4）的动力学和抗ige剂量反应参数被表征。介质的相对释放速率组胺大于PGD2 = TxB2大于LTC4，最大释放时间分别约为2、5和10 min。在2个实验中，抗ige刺激导致大量血小板活化因子（PAF）迅速（2分钟）出现在细胞颗粒中；细胞相关PAF在45分钟后下降到低水平。花生四烯酸环加氧酶代谢产物PGD2和TxB2的最佳释放浓度（0.3微克/毫升）比组胺和LTC4的释放浓度（3微克/毫升）低10- 30倍，表明这些释放过程可能对IgE Fc受体交联有不同的要求。在最佳组胺释放时，发现每种花生四烯酸代谢物的释放量与组胺释放量相对应，然而，这表明这两个过程是串联或平行联系的。
The release of inflammatory mediators (histamine, PGD2, TxB2, and LTC4) from purified human lung mast cells was characterized by kinetic and anti-IgE dose-response parameters. The relative rate of mediator release was histamine greater than PGD2 = TxB2 greater than LTC4, with one half maximal release occurring at approximately 2, 5, and 10 min, respectively. In 2 experiments, stimulation with anti-IgE caused significant quantities of platelet-activating factor (PAF) to appear rapidly (2 min) in the cell pellet; cell-associated PAF declined to low levels by 45 min. The optimal concentration of anti-IgE for the release of the arachidonate cyclooxygenase metabolites PGD2 and TxB2 (0.3 microgram/ml) was 10- to 30-fold less than that required for the release of histamine and LTC4 (3 to 10 micrograms/ml), suggesting that these release processes may have differential IgE Fc receptor cross-linking requirements. At optimal histamine release, the magnitude of the release of each arachidonate metabolite was found to correspond to the magnitude of histamine release, however, suggesting that the 2 processes are linked either in series or in parallel.