Arginine supplementation improves rotorod performance in sickle transgenic mice.
Arginine supplementation improves rotorod performance in sickle transgenic mice.
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DOI：
10.1080/10245330410001714185
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发表时间：
2004-08-01
期刊：
影响因子：
--
通讯作者：
Fabry, Mary E
中科院分区：
文献类型：
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作者：
Fasipe, Francisca R;Ubawike, Adaeze E;Fabry, Mary E
镰状细胞病（SCD）患者已被证明有视觉运动速度和协调受损。小鼠的感觉运动缺陷可以通过运动协调测试来研究，这种测试需要全身运动，如旋转杆。将表达人α、人β (S)和人β (S- antilles)的镰刀型转基因小鼠模型（S+S- antilles）与对照C57BL/6J小鼠和rotorod上添加5%精氨酸的S+S- antilles小鼠相比，小鼠β（主要）缺失为纯合子，血浆精氨酸水平较低。旋转杆由直径2.5厘米的沟槽杆组成，以恒定的加速度转动，需要小鼠调整姿势以保持平衡。Purina小鼠的C57BL小鼠的平均下降潜伏期为S+S- antilles小鼠，Purina小鼠的平均下降潜伏期为127+/-56 S， S+S- antilles小鼠补充5%精氨酸后的平均下降潜伏期可通过增加NO合成从而改善血管舒张和血流，从而逆转脑和/或肌肉缺血来改善镰刀转基因小鼠的旋转性能。总之，镰状转基因小鼠的旋转功能受损提供了一个机会，可以应用这个简单的任务来提供一种有效的方法来筛选某些类型的治疗方案对SCD的疗效。
Patients with sickle cell disease (SCD) have been shown to have impaired visual-motor speed and coordination. Sensorimotor deficits in mice can be investigated by motor coordination tests that require whole body movements such as the rotorod. A sickle transgenic mouse model (S+S-Antilles) that expresses human alpha, human beta(S) and human beta(S-Antilles), is homozygous for the mouse beta(major) deletion, and has low plasma arginine was compared to control C57BL/6J mice and S+S-Antilles mice supplemented with 5% arginine on the rotorod. The rotorod consists of a 2.5 cm diameter, grooved rod turning at constant acceleration, requiring postural adjustments on the part of the mice to maintain equilibrium. C57BL mice on Purina mouse chow had an average latency to fall of S+S-Antilles mice on Purina mouse chow had an average of 127+/-56 s S+S-Antilles mice after 5% arginine supplementation had a mean latency of Arginine may improve rotorod performance in sickle transgenic mice by increasing NO synthesis thereby improving vasodilatation and blood flow with reversal of ischemia in brain and/or muscle. In conclusion, impaired rotorod performance in sickle transgenic mice presents an opportunity to apply this simple task to provide an efficient method to screen some types of therapeutic regimens for efficacy in SCD.