Comparison of immune microenvironments between primary tumors and brain metastases in patients with breast cancer.
Comparison of immune microenvironments between primary tumors and brain metastases in patients with breast cancer.
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DOI：
10.18632/oncotarget.22110
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发表时间：
2017-11-28
期刊：
影响因子：
--
通讯作者：
Iwata H
中科院分区：
文献类型：
--
作者：
Ogiya R;Niikura N;Kumaki N;Yasojima H;Iwasa T;Kanbayashi C;Oshitanai R;Tsuneizumi M;Watanabe KI;Matsui A;Fujisawa T;Saji S;Masuda N;Tokuda Y;Iwata H
据报道，免疫检查点抑制剂对脑转移患者有效。然而，脑转移瘤免疫微环境的详细特征仍未被探索。脑转移瘤中的中位肿瘤浸润淋巴细胞（TIL）类别为5%（1-70%）。在46例配对标本中，乳腺原发肿瘤的TIL百分比显著高于脑转移瘤（配对t检验，P < 0.01）。原发性乳腺肿瘤中CD 4/CD 8/Foxp 3阳性细胞的数量显著高于脑转移瘤（配对t检验，所有抗体P < 0.05）。特别是在三阴性乳腺癌患者中，与高TIL数量相比，低TIL数量与显著较短的总生存期相关（对数秩检验，P = 0.04）。我们回顾性地确定了2001年至2012年在8家机构接受手术的107例乳腺癌和脑转移患者，并收集了191份样本，包括单独的脑转移瘤和原发性肿瘤与配对的脑转移瘤样本。对苏木精和伊红染色的载玻片进行TIL评价，并根据染色程度进行分类。还进行了CD 4、CD 8、Foxp 3、PD-L1、PD-L2和HLA I类的免疫组织化学。脑转移瘤中的肿瘤内淋巴细胞数量明显少于原发性乳腺肿瘤。
Immune checkpoint inhibitors are reported to be effective in patients with brain metastases. However, detailed characteristics of the brain metastasis immune microenvironment remain unexplored. The median tumor-infiltrating lymphocyte (TIL) category in brain metastases was 5% (1–70%). In 46 pair-matched samples, the percentages of TILs were significantly higher in primary breast tumors than in brain metastases (paired t-test, P < 0.01). The numbers of CD4/CD8/Foxp3-positive cells were significantly higher in primary breast tumors than in brain metastases (paired t-test, P < 0.05 for all antibodies). In patients with triple-negative breast cancer specifically, low TIL numbers were associated with significantly shorter overall survival compared to high TIL numbers (log-rank test, P = 0.04). We retrospectively identified 107 patients with breast cancer and brain metastases who had undergone surgery between 2001 and 2012 at 8 institutions, and collected 191 samples including brain metastases alone and primary tumors with pair-matched brain metastasis samples. Hematoxylin and eosin-stained slides were evaluated for TILs and categorized according to the extent of staining. Immunohistochemistry for CD4, CD8, Foxp3, PD-L1, PD-L2, and HLA class I was also performed. There are significantly fewer TILs in brain metastases than in primary breast tumors.
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DOI：
10.1007/s10555-016-9652-y
发表时间：
2017-03
期刊：
Cancer metastasis reviews
影响因子：
--
作者：
Khagi Y;Kurzrock R;Patel SP
通讯作者：
Patel SP
DOI：
10.1016/s1470-2045(16)30053-5
发表时间：
2016-07
期刊：
The Lancet. Oncology
影响因子：
--
作者：
Goldberg SB;Gettinger SN;Mahajan A;Chiang AC;Herbst RS;Sznol M;Tsiouris AJ;Cohen J;Vortmeyer A;Jilaveanu L;Yu J;Hegde U;Speaker S;Madura M;Ralabate A;Rivera A;Rowen E;Gerrish H;Yao X;Chiang V;Kluger HM
通讯作者：
Kluger HM
影响因子：
50.3
作者：
Tang H;Wang Y;Chlewicki LK;Zhang Y;Guo J;Liang W;Wang J;Wang X;Fu YX
通讯作者：
Fu YX
影响因子：
45.3
作者：
Nanda, Rita;Chow, Laura Q. M.;Buisseret, Laurence
通讯作者：
Buisseret, Laurence
影响因子：
--
作者：
Harter PN;Bernatz S;Scholz A;Zeiner PS;Zinke J;Kiyose M;Blasel S;Beschorner R;Senft C;Bender B;Ronellenfitsch MW;Wikman H;Glatzel M;Meinhardt M;Juratli TA;Steinbach JP;Plate KH;Wischhusen J;Weide B;Mittelbronn M
通讯作者：
Mittelbronn M