Membrane locus and pH sensitivity of paraben inhibition of alkali production by oral streptococci.
Membrane locus and pH sensitivity of paraben inhibition of alkali production by oral streptococci.
复制标题
对羟基苯甲酸酯抑制口腔链球菌产碱的膜位点和 pH 敏感性。
DOI：
10.1034/j.1399-302x.1999.140408.x
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发表时间：
1999
影响因子：
--
通讯作者：
Marquis,RE
中科院分区：
文献类型：
--
作者：
Ma,Y;Rutherford,GC;Curran,TM;Reidmiller,JS;Marquis,RE
对羟基苯甲酸酯被发现是有效的抑制精氨酸产生碱的口服链球菌，如家鼠链球菌，血链球菌和戈登链球菌。例如，每ml 2 μmol对羟基苯甲酸丁酯完全不可逆地抑制s。rattusFA‐1，对该生物是致命的。相反，对羟基苯甲酸丁酯并不是一种非常有效的唾液链球菌完整细胞溶尿抑制剂。我，虽然它确实杀死了细胞。对羟基苯甲酸丁酯不可逆地抑制胞质酶精氨酸脱亚胺酶，氨基甲酸酯激酶和脲酶在渗透细胞或分离形式。然而，完整细胞对精氨酸分解的抑制似乎主要是由于对精氨酸摄取的运输系统的不可逆抑制，因为在完整细胞中添加对羟基苯甲酸丁酯并没有降低洗涤后细胞渗透后精氨酸脱亚胺酶的水平。完整细胞对羟基苯甲酸丁酯的不敏感可能与已知的细胞膜对尿素的高通透性和脲酶的细胞质位置有关。对羟基苯甲酸丁酯作为精氨酸溶解或糖酵解的抑制剂和致死剂的效力在酸性pH值下比在中性或碱性pH值下更大。
Parabens were found to be potent inhibitors of alkali production from arginineby oral streptococci such asStreptococcus rattus,Streptococcus sanguisandStreptococcus gordonii. For example, 2 μmol butylparaben per ml completely and irreversibly inhibited arginolysis by intact cells ofS. rattusFA‐1 and was lethal for the organism. In contrast, butylparaben was not a very effective inhibitor of ureolysis by intact cells ofStreptococcus salivarius57.I, although it did kill the cells. Butylparaben irreversibly inhibited the cytoplasmic enzymes arginine deiminase, carbamate kinase and urease in permeabilized cells or isolated form. However, inhibition of arginolysis by intact cells appeared to be due primarily to irreversible inhibition of transport systems for arginine uptake, because butylparaben added to intact cells did not reduce levels of arginine deiminase when the cells were subsequently permeabilized after washing. The insensitivity of ureolysis by intact cells to butylparaben can be related to the known high permeability of cell membranes to urea and the cytoplasmic location of urease. The potency of butylparaben as an inhibitior of arginolysis or glycolysis and as a lethal agent was found to be greater at acid pH that at neutral or alkaline pH.