Visualization and exploration of Tcf/Lef function using a highly responsive Wnt/β-catenin signaling-reporter transgenic zebrafish
Visualization and exploration of Tcf/Lef function using a highly responsive Wnt/β-catenin signaling-reporter transgenic zebrafish
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DOI：
10.1016/j.ydbio.2012.07.016
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发表时间：
2012-10-01
影响因子：
2.7
通讯作者：
Ishitani, Tohru
中科院分区：
文献类型：
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作者：
Shimizu, Nobuyuki;Kawakami, Koichi;Ishitani, Tohru
进化上保守的Tcf/Lef转录因子(Lef1、Tcf7、Tcf711和Tcf712)通过Wnt/β-catenin信号调节基因表达，在早期胚胎发生、器官发生、成人组织动态平衡和组织再生中发挥多种作用。然而，在这些事件期间Tcf/LEF活动的时空动力学仍然知之甚少。我们获得了稳定的转基因斑马鱼品系，携带新的Wnt/β-catenin信号报告Tcf/Lef-mini P：dGFP。记者揭示了四个受Wnt/β-catenin信号调控的Tcf/Lef成员的转录活性，它们在胚胎发生、器官发育和生长以及组织再生过程中表达在已知的Wnt/β-catenin信号活性部位。我们使用转基因系来证明Tcf/Lef介导的Wnt/β-catenin信号对前侧线、背侧和次级后侧线以及鳃细丝的发育起到了作用。因此，这些报告线对于研究Tcf/Lef介导的Wnt/β-catenin信号依赖过程是非常有用的工具。(C)2012 Elsevier Inc.保留所有权利。
Evolutionarily conserved Tcf/Lef transcription factors (Lef1, Tcf7, Tcf711, and Tcf712) mediate gene expression regulated by Wnt/beta-catenin signaling, which has multiple roles in early embryogenesis, organogenesis, adult tissue homeostasis, and tissue regeneration. However, the spatiotemporal dynamics of Tcf/Lef activity during these events remain poorly understood. We generated stable transgenic zebrafish lines carrying a new Wnt/beta-catenin signaling reporter, Tcf/Lef-miniP:dGFP. The reporter revealed the transcriptional activities of four Tcf/Lef members controlled by Wnt/beta-catenin signaling, which were expressed in known Wnt/beta-catenin signaling-active sites during embryogenesis, organ development and growth, and tissue regeneration. We used the transgenic lines to demonstrate the contribution of Tcf/Lef-mediated Wnt/beta-catenin signaling to the development of the anterior lateral line, dorsal and secondary posterior lateral lines, and gill filaments. Thus, these reporter lines are highly useful tools for studying Tcf/Lef-mediated Wnt/beta-catenin signaling-dependent processes. (C) 2012 Elsevier Inc. All rights reserved.