Comparative Analysis of Cartilage Marker Gene Expression Patterns during Axolotl and Xenopus Limb Regeneration.
Comparative Analysis of Cartilage Marker Gene Expression Patterns during Axolotl and Xenopus Limb Regeneration.
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DOI：
10.1371/journal.pone.0133375
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Satoh A
中科院分区：
文献类型：
--
作者：
Mitogawa K;Makanae A;Satoh A;Satoh A
蝾螈（Ambystoma mexicanum）可以完全再生失去的肢体，而非洲爪蟾蛙不能。在肢体再生过程中，芽基首先在截肢平面形成。据认为，这种再生芽基通过与发育中的胚胎肢芽相似的机制形成肢。此外，非洲爪蟾蛙在截肢后可以形成芽基;然而，芽基导致一个末端锥形软骨结构，称为“穗”。这种图案缺陷的原因在爪蟾蛙肢体再生进行了探讨。我们假设软骨形成的差异可能是图案缺陷的基础。因此，我们专注于软骨形成。软骨发生标记基因，I型和II型胶原蛋白，在再生和非再生环境进行了比较。有显着差异蝾螈和爪蟾在这些软骨发生相关基因的表达模式。非洲爪蟾胚基细胞的软骨形成能力的相对不足，可能是导致其完全肢体再生能力缺失的原因。
Axolotls (Ambystoma mexicanum) can completely regenerate lost limbs, whereas Xenopus laevis frogs cannot. During limb regeneration, a blastema is first formed at the amputation plane. It is thought that this regeneration blastema forms a limb by mechanisms similar to those of a developing embryonic limb bud. Furthermore, Xenopus laevis frogs can form a blastema after amputation; however, the blastema results in a terminal cone-shaped cartilaginous structure called a “spike.” The causes of this patterning defect in Xenopus frog limb regeneration were explored. We hypothesized that differences in chondrogenesis may underlie the patterning defect. Thus, we focused on chondrogenesis. Chondrogenesis marker genes, type I and type II collagen, were compared in regenerative and nonregenerative environments. There were marked differences between axolotls and Xenopus in the expression pattern of these chondrogenesis-associated genes. The relative deficit in the chondrogenic capacity of Xenopus blastema cells may account for the absence of total limb regenerative capacity.