Vasoactive intestinal polypeptide: a putative transmitter in the canine gastric muscularis mucosa.
Vasoactive intestinal polypeptide: a putative transmitter in the canine gastric muscularis mucosa.
复制标题
血管活性肠多肽：犬胃粘膜肌层中的假定递质。
DOI：
10.1113/jphysiol.1983.sp014830
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
Szurszewski,JH
中科院分区：
文献类型：
--
作者：
Angel,F;Go,VL;Schmalz,PF;Szurszewski,JH
采用灌流技术研究了离体犬胃粘膜肌层抑制性递质的性质。神经刺激（10 V，200 μ s，10 Hz）的抑制作用不改变肾上腺素能，胆碱能或胆碱能拮抗剂。三磷酸腺苷对自发性机械活动无影响。核苷酸焦磷酸酶和蜂毒肽对神经刺激的反应没有影响。α-糜蛋白酶消除了神经刺激的抑制作用。肌肉的放射免疫测定表明存在胃泌素/胆囊收缩素-P物质-和血管活性肠多肽（VIP）样免疫反应性。在存在的三种肽中，仅VIP产生浓度依赖性松弛。在神经诱导的肌肉松弛过程中释放了一种具有VIP样免疫反应性的物质，其释放被河豚毒素和去钙溶液阻断。VIP抗血清可消除神经刺激的抑制作用。这些数据强烈支持的假设，VIP或密切相关的肽是一种抑制性神经递质在犬胃粘膜肌层。
The nature of the inhibitory transmitter in the canine gastric muscularis mucosae was studied in vitro using superfusion techniques. The inhibitory effect of nerve stimulation (10 V, 200 mus, 10 Hz) was not altered by adrenergic, cholinergic or serotonergic antagonists. Adenosine triphosphate had no effect on spontaneous mechanical activity. Nucleotide pyrophosphatase and apamin had no effect on the response to nerve stimulation. Alpha‐chymotrypsin abolished the inhibitory effect of nerve stimulation. Radioimmunoassay of the muscle indicated the presence of gastrin/cholecystokinin‐substance P‐ and vasoactive intestinal polypeptide (VIP)‐like immunoreactivity. Of the three peptides present, only VIP produced a concentration‐dependent relaxation. A substance with VIP‐like immunoreactivity was released during nerve‐induced relaxation of the muscle, and its release was blocked by tetrodotoxin and calcium‐depleted solution. The inhibitory effect of nerve stimulation was abolished by VIP antiserum. These data strongly support the hypothesis that VIP or a closely related peptide is an inhibitory neurotransmitter in the canine gastric muscularis mucosae.