Dynamics of Salmonella infection of macrophages at the single cell level.
Dynamics of Salmonella infection of macrophages at the single cell level.
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DOI：
10.1098/rsif.2012.0163
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发表时间：
2012-10-07
期刊：
影响因子：
--
通讯作者：
Bryant CE
中科院分区：
文献类型：
--
作者：
Gog JR;Murcia A;Osterman N;Restif O;McKinley TJ;Sheppard M;Achouri S;Wei B;Mastroeni P;Wood JL;Maskell DJ;Cicuta P;Bryant CE
肠道沙门氏菌会引起一系列疾病。沙门氏菌是巨噬细胞的细胞内寄生虫，控制这些细胞内的细菌对于抵抗感染至关重要。细菌入侵、生存、在巨噬细胞中增殖和杀死巨噬细胞的动态是疾病发病机制的核心。然而，诸如细胞感染率之类的基本重要参数此前并未被计算过。我们使用两种独立的方法来计算巨噬细胞感染率：沙门氏菌感染实验的数学模型和感染事件的实时视频显微镜分析。细胞反复遇到沙门氏菌，细菌通常与巨噬细胞结合十秒以上。一旦沙门氏菌遇到巨噬细胞，该细菌感染细胞的概率就非常低：低于 5%。巨噬细胞群对首次感染事件的易感性是异质的。一旦被感染，巨噬细胞可以经历进一步的感染事件，但这些再感染事件的发生率低于初次感染的发生率。
Salmonella enterica causes a range of diseases. Salmonellae are intracellular parasites of macrophages, and the control of bacteria within these cells is critical to surviving an infection. The dynamics of the bacteria invading, surviving, proliferating in and killing macrophages are central to disease pathogenesis. Fundamentally important parameters, however, such as the cellular infection rate, have not previously been calculated. We used two independent approaches to calculate the macrophage infection rate: mathematical modelling of Salmonella infection experiments, and analysis of real-time video microscopy of infection events. Cells repeatedly encounter salmonellae, with the bacteria often remain associated with the macrophage for more than ten seconds. Once Salmonella encounters a macrophage, the probability of that bacterium infecting the cell is remarkably low: less than 5%. The macrophage population is heterogeneous in terms of its susceptibility to the first infection event. Once infected, a macrophage can undergo further infection events, but these reinfection events occur at a lower rate than that of the primary infection.
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影响因子：
23.8
作者：
HOLLING, CS
通讯作者：
HOLLING, CS
影响因子：
5.8
作者：
Gorvel, JP;Méresse, S
通讯作者：
Méresse, S
影响因子：
4.8
作者：
Alldridge, LC;Harris, HJ;Bryant, CE
通讯作者：
Bryant, CE
DOI：
10.1084/jem.176.1.287
发表时间：
1992-07-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Stein M;Keshav S;Harris N;Gordon S
通讯作者：
Gordon S
DOI：
10.1073/pnas.1000041107
发表时间：
2010-02-23
影响因子：
11.1
作者：
Helaine, Sophie;Thompson, Jessica A.;Holden, David W.
通讯作者：
Holden, David W.