Acoustic stiffness and change in plug cartilage over time after autologous osteochondral grafting: correlation between ultrasound signal intensity and histological score in a rabbit model.
Acoustic stiffness and change in plug cartilage over time after autologous osteochondral grafting: correlation between ultrasound signal intensity and histological score in a rabbit model.
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DOI：
10.1186/ar1219
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发表时间：
2004
影响因子：
4.9
通讯作者：
Nakamura, Takashi
中科院分区：
文献类型：
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作者：
Kuroki, Hiroshi;Nakagawa, Yasuaki;Mori, Koji;Ohba, Mao;Suzuki, Takashi;Mizuno, Yasuyuki;Ando, Keiji;Takenaka, Makoto;Ikeuchi, Ken;Nakamura, Takashi
我们研究了自体骨软骨移植动物模型中软骨栓的超声信号强度（硬度指标）、信号持续时间（表面不规则性指标）和信号间隔（厚度指标）随时间的定量变化。通过手术切除并替换雄性日本白兔（n = 22）的全层骨软骨塞。使用超声系统以及宏观和组织学评估（改良的Mankin评分）对术后第0天和第2、4、8、12和24周获得的标本进行评估。组织学显示，直到术后 2 周，栓塞才下沉，新形成的软骨样组织覆盖栓塞，但在 24 周时，组织脱落。在整个观察期间，塞子本身完好无损。尽管第0天时插塞部位的信号强度与假手术对侧膝关节的信号强度相同，但术后2至24周，信号强度低于假手术膝关节的信号强度。 8 周时，这种差异显着（P < 0.05）。修改后的曼金评分显示该部位存在早期退行性变化，但肉眼检查并未发现。信号强度与评分显着相关（第 0 天和术后五个时间点 [P < 0.05，r = -0.91] 以及整体 [P < 0.05，r = -0.36]）。信号强度还与修改后的 Mankin 评分中的“软骨结构”（P < 0.05，r = -0.32）和“软骨细胞”（P < 0.05，r = -0.30）的各个子评分显着相关，但与“染色”和“潮痕”的子评分不显着相关。信号持续时间与总分显着相关（总体上[P < 0.05，r = 0.34]），但与软骨结构得分不显着相关（P = 0.0557，r = 0.29）。信号之间的间隔很好地反映了塞点的实际厚度。超声信号强度和评分之间的显着关系表明，软骨栓和软骨样组织（尤其是浅层）的早期退行性变化可以通过高频超声评估来检测。
We investigated quantitative changes over time in ultrasound signal intensity (an index of stiffness), signal duration (an index of surface irregularity), and interval between signals (an index of thickness) of plug cartilage in an animal model of autologous osteochondral grafting. A full-thickness osteochondral plug was surgically removed and replaced in male Japanese white rabbits (n = 22). Specimens obtained at day 0 and weeks 2, 4, 8, 12 and 24 postoperatively were assessed using an ultrasound system and by macroscopic and histological evaluation (modified Mankin's score). Histology revealed that the plug sank until 2 weeks postoperatively, and that newly formed cartilage-like tissue covered the plug, but at 24 weeks the tissue detached. The plug itself survived well throughout the period of observation. Although the signal intensity at the plug site was same as that in the sham operated contralateral knee at day 0, from 2 to 24 weeks postoperatively it was less than that in the sham knee. At 8 weeks, this difference was significant (P < 0.05). Modified Mankin's score revealed early degenerative changes at the site, but macroscopic examination did not. Signal intensity correlated significantly with score (both at day 0 and at the five postoperative time points [P < 0.05, r = -0.91] and as a whole [P < 0.05, r = -0.36]). Signal intensity also significantly correlated with the individual subscores for 'cartilage structure' (P < 0.05, r = -0.32) and 'cartilage cells' (P < 0.05, r = -0.30) from the modified Mankin's score, but not significantly with subscores for 'staining' and 'tidemark'. Signal duration correlated significantly with total score (as a whole [P < 0.05, r = 0.34]), but not significantly with the score for cartilage structure (P = 0.0557, r = 0.29). The interval between signals reflected well the actual thickness of the plug site. The significant relationships between ultrasound signal intensity and scores suggest that early degenerative changes in plug cartilage and cartilage-like tissue, especially in the superficial layer, are detectable by high-frequency ultrasound assessment.