Assembly of hepatitis delta virus particles.
Assembly of hepatitis delta virus particles.
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丁型肝炎病毒颗粒的组装。
DOI：
10.1128/jvi.66.4.2310-2315.1992
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发表时间：
1992
影响因子：
5.4
通讯作者：
Taylor,J
中科院分区：
文献类型：
--
作者：
Ryu,WS;Bayer,M;Taylor,J
丁型肝炎病毒（HDV）是乙型肝炎病毒（HBV）的一种亚病毒卫星病毒。由于HDV的RNA基因组可以在没有HBV的情况下在培养细胞中复制，因此有人认为HBV的唯一辅助功能是在HDV颗粒的组装过程中提供HBV外壳蛋白。为了研究参与这种病毒粒子组装的因素，我们短暂地用各种肝病毒构建体和HDV cdna共转染细胞，并分析释放到培养基中的颗粒。我们报道了HDV基因组RNA和丁型抗原可以被HBV或相关的肝病毒土旱獭肝炎病毒（WHV）的外壳蛋白包装。在WHV的3个共羧基末端外壳蛋白中，最小的形式足以包装HDV基因组；即使在没有HDV RNA的情况下，丁型抗原也可以被这种WHV外壳蛋白包裹。此外，两种共氨基末端形式的三角抗原，只有较大的形式是必要的包装。
Hepatitis delta virus (HDV) is a subviral satellite of hepatitis B virus (HBV). Since the RNA genome of HDV can replicate in cultured cells in the absence of HBV, it has been suggested that the only helper function of HBV is to supply HBV coat proteins in the assembly process of HDV particles. To examine the factors involved in such virion assembly, we transiently cotransfected cells with various hepadnavirus constructs and cDNAs of HDV and analyzed the particles released into the medium. We report that the HDV genomic RNA and the delta antigen can be packaged by coat proteins of either HBV or the related hepadnavirus woodchuck hepatitis virus (WHV). Among the three co-carboxy-terminal coat proteins of WHV, the smallest form was sufficient to package the HDV genome; even in the absence of HDV RNA, the delta antigen could be packaged by this WHV coat protein. Also, of the two co-amino-terminal forms of the delta antigen, only the larger form was essential for packaging.