Rice Gene Targeting by Homologous Recombination with Positive-Negative Selection Strategy
Rice Gene Targeting by Homologous Recombination with Positive-Negative Selection Strategy
复制标题
采用正负选择策略的同源重组水稻基因打靶
DOI：
10.1007/978-1-0716-1068-8_16
复制
发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Shimatani Zenpei
中科院分区：
文献类型：
--
作者：
Terada Rie;Shimatani Zenpei
基因打靶（Gene targeting，GT）是通过同源重组（homologous recombination，HR）改变基因组中特定基因的结构。它在功能基因组学中起着重要的作用，因为它能够将内源基因精确修饰成所需的形式，例如敲除、敲入、引入点突变以及产生融合基因。此外，GT定点突变也可以作为一种优秀的技术应用于分子育种和基因治疗，因为它可以直接反映从功能基因组学获得的知识。在本节中，我们介绍了成熟的GT程序在水稻结合阳性-阴性选择（PNS）策略。
Gene targeting (GT) is a technique that alter the structure of the specific genes at their original loci in the genome by homologous recombination (HR). It plays an important role in functional genomics because it enables precise modification of the endogenous genes into desired forms such as knockout, knock-in, introduction of point mutations, as well as generation of fusion genes. Also, site-directed mutagenesis by GT can also be applied as an excellent technique for molecular breeding and gene therapy, because it can directly reflect the knowledge acquired from functional genomics. In this section, we introduce well-established GT procedure in rice in combination with positive-negative-selection (PNS) strategy.