DIFFERENTIATION AND CHARACTERIZATION OF AUTOANTIBODIES AND THEIR ANTIGENS IN SJOGRENS SYNDROME
DIFFERENTIATION AND CHARACTERIZATION OF AUTOANTIBODIES AND THEIR ANTIGENS IN SJOGRENS SYNDROME
复制标题
DOI：
10.1002/art.1780190214
复制
发表时间：
1976-01-01
影响因子：
--
通讯作者：
TAN, EM
中科院分区：
文献类型：
--
作者：
ALSPAUGH, MA;TALAL, N;TAN, EM
应用免疫扩散法检测干燥综合征（SS）自身抗体与组织抗原的沉淀反应。抗原的来源是在组织培养中生长的人淋巴样细胞（WiI 2）的超声处理提取物。不同的沉淀素系统（3）被鉴定并称为沉淀素SS-A和SS-B，以及类风湿性关节炎沉淀素（RAP）。在没有相关类风湿性关节炎（RA）的SS患者的血清中存在高频率的维生素SS-A和SS-B，并且在许多其他结缔组织疾病中不存在或以低频率存在。具有与RA相容的临床特征的SS患者（SS-RA）不具有沉淀素SS-A和SS-B，但具有RAP。后者沉淀素也存在于类风湿关节炎患者中，频率顺序与SS-RA相似。3种沉淀素系统均为免疫球蛋白与细胞抗原之间的反应。SS-A和SS-B抗原的起源被证明是主要从细胞核免疫荧光，但RAP在细胞中的起源没有建立。
Autoantibodies in Sjogrens syndrome (SS) were identified by their precipitin reactions with tissue antigens in immunodiffusion. The source of antigens was a sonicated extract of human lymphoid cells (WiI2) grown in tissue culture. Distinct precipitin systems (3) were identified and referred to as precipitins SS-A and SS-B, and rheumatoid arthritis precipitin (RAP). Precipitins SS-A and SS-B are present in high frequency in sera of patients with SS without associated rheumatoid arthritis (RA), and are absent or present in low frequency in many other connective tissue diseases. Patients with SS who had clinical features compatible with RA (SS-RA) did not have precipitins SS-A and SS-B, but had RAP. The latter precipitin was also present in RA patients in a similar order of frequency as in SS-RA. All 3 precipitin systems were reactions between immunoglobulins and cellular antigens. The origin of SS-A and SS-B antigens was demonstrated by immunofluorescence to be primarily from the nucleus, but the origin of RAP in the cell was not established.