SENSITIVITY, POLARITY, AND CONDUCTANCE CHANGE IN RESPONSE OF VERTEBRATE HAIR CELLS TO CONTROLLED MECHANICAL STIMULI
SENSITIVITY, POLARITY, AND CONDUCTANCE CHANGE IN RESPONSE OF VERTEBRATE HAIR CELLS TO CONTROLLED MECHANICAL STIMULI
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DOI：
10.1073/pnas.74.6.2407
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发表时间：
1977-01-01
影响因子：
11.1
通讯作者：
COREY, DP
中科院分区：
文献类型：
--
作者：
HUDSPETH, AJ;COREY, DP
毛细胞是听觉、前庭和侧线感觉系统的主要受体，可响应顶端毛束的机械刺激而产生电信号。采用体外制备和细胞内记录研究牛蛙（Rana catesbeiana）内耳球囊毛细胞的转导机制。当直接通过发束的机械偏转刺激时，这些细胞会产生幅度高达 15 mV 的分级反应；峰值灵敏度约为 20 mV/μm 偏转。受体电位的去极化成分对应于针对运动纤毛的刺激。去极化反应与膜电阻降低相关，超极化反应与膜电阻增加相关。机械或电刺激偶尔会在毛细胞中诱发动作电位，可能是 C2+ 尖峰。
Hair cells, the primary receptors of the auditory, vestibular, and lateral-line sensory systems, produce electrical signals in response to mechanical stimulation of their apical hair bundles. An in vitro preparation and intracellular recording were employed to investigate the transduction mechanism of hair cells in the sacculus from the inner ear of the bullfrog (Rana catesbeiana). When stimulated directly by mechanical deflection of their hair bundles, these cells gave graded responses up to 15 mV in amplitude; the peak sensitivity was about 20 mV/.mu.m deflection. The depolarizing component of the receptor potential corresponded to stimuli directed towards the kinocilium. Depolarizing responses were associated with a membrane resistance decrease, the hyperpolarizing responses with a resistance increase. Action potentials, possibly C2+ spikes, were occasionally evoked in hair cells by mechanical or electrical stimulation.