Optimization and characterization of biosurfactant production from marine Vibrio sp. strain 3B-2.
Optimization and characterization of biosurfactant production from marine Vibrio sp. strain 3B-2.
复制标题
海洋弧菌生物表面活性剂生产的优化和表征。
DOI：
10.3389/fmicb.2015.00976
复制
发表时间：
2015
影响因子：
5.2
通讯作者：
Wang P
中科院分区：
文献类型：
--
作者：
Hu X;Wang C;Wang P
从海洋沉积物中分离到一株产生物表面活性剂的细菌，命名为3B-2，经16 S rRNA基因测序鉴定为弧菌属（Vibrio sp.）。对3B-2菌株的培养基组成进行了优化，以提高其产生物表面活性剂的能力。所产生的生物表面活性剂的特征在于在蛋白质浓度，表面张力，和驱油效率。最佳培养基组成为：乳糖0.5%，酵母膏1.1%，氯化钠2%，磷酸氢二钠0.1%。在最佳条件下（28°C），生物表面活性剂粗品的表面张力可从71.5mN/m（水）降至41，蛋白质浓度可达6.5g/L，驱油效率在6.5cm处有显著提高。从该生物表面活性剂中分离纯化了分子量为22和40 kDa的两种糖蛋白组分，在微生物采油和生物修复中具有很大的应用潜力。
A biosurfactant-producing bacterium, designated 3B-2, was isolated from marine sediment and identified as Vibrio sp. by 16S rRNA gene sequencing. The culture medium composition was optimized to increase the capability of 3B-2 for producing biosurfactant. The produced biosurfactant was characterized in terms of protein concentration, surface tension, and oil-displacement efficiency. The optimal medium for biosurfactant production contained: 0.5% lactose, 1.1% yeast extract, 2% sodium chloride, and 0.1% disodium hydrogen phosphate. Under optimal conditions (28°C), the surface tension of crude biosurfactant could be reduced to 41 from 71.5 mN/m (water), while its protein concentration was increased to up to 6.5 g/L and the oil displacement efficiency was improved dramatically at 6.5 cm. Two glycoprotein fractions with the molecular masses of 22 and 40 kDa were purified from the biosurfactant, which held great potential for applications in microbial enhanced oil recovery and bioremediation.