A molecularly imprinted polymer nanoparticle-based surface plasmon resonance sensor platform for antibiotic detection in river water and milk.
A molecularly imprinted polymer nanoparticle-based surface plasmon resonance sensor platform for antibiotic detection in river water and milk.
复制标题
基于分子印迹聚合物纳米颗粒的表面等离子共振传感器平台，用于河水和牛奶中的抗生素检测。
DOI：
10.1007/s00216-022-04012-8
复制
发表时间：
2022
影响因子：
4.3
通讯作者：
Sullivan MV
中科院分区：
文献类型：
--
作者：
Sullivan MV
利用固相分子印迹技术，合成了对目标抗生素环丙沙星、阿托沙星和氧氟沙星具有选择性的高亲和性纳米粒子。这些已被应用于开发的表面等离子体共振（SPR）传感器检测河水和牛奶中的三种抗生素。所产生的颗粒表现出良好的均匀性，具有65.8 ± 1.8 nm、76.3 ± 4.1 nm和85.7 ± 2.5 nm的近似尺寸，并且表现出分别对环丙沙星、诺氟沙星和氧氟沙星纳米MIP具有36.2 nM、54.7 nM和34.6 nM的亲和力。交叉反应性研究强调了与非靶标抗生素相比，对靶标抗生素的良好选择性。使用加标的牛奶和河水样品，基于nanoMIP的SPR传感器提供了与66.8 nM，33.4 nM和55.0 nM（牛奶）和39.3 nM，26.1 nM和42.7 nM（河水）的环丙沙星，诺氟沙星和氧氟沙星nanoMIP的亲和力相当，分别在缓冲液标准中观察到。牛奶和河水中三种抗生素靶标的估计LOD均为低nM或更低。所开发的SPR传感器显示出良好的潜力，使用该技术捕获和检测食品和环境样品中的抗生素。图形摘要
Using a solid-phase molecular imprinting technique, high-affinity nanoparticles (nanoMIPs) selective for the target antibiotics, ciprofloxacin, moxifloxacin, and ofloxacin have been synthesised. These have been applied in the development of a surface plasmon resonance (SPR) sensor for the detection of the three antibiotics in both river water and milk. The particles produced demonstrated good uniformity with approximate sizes of 65.8 ± 1.8 nm, 76.3 ± 4.1 nm, and 85.7 ± 2.5 nm, and were demonstrated to have affinities of 36.2 nM, 54.7 nM, and 34.6 nM for the ciprofloxacin, moxifloxacin, and ofloxacin nanoMIPs, respectively. Cross-reactivity studies highlighted good selectivity towards the target antibiotic compared with a non-target antibiotic. Using spiked milk and river water samples, the nanoMIP-based SPR sensor offered comparable affinity with 66.8 nM, 33.4 nM, and 55.0 nM (milk) and 39.3 nM, 26.1 nM, and 42.7 nM (river water) for ciprofloxacin, moxifloxacin, and ofloxacin nanoMIPs, respectively, to that seen within a buffer standard. Estimated LODs for the three antibiotic targets in both milk and river water were low nM or below. The developed SPR sensor showed good potential for using the technology for the capture and detection of antibiotics from food and environmental samples.Graphical abstract