Transforming growth factor β1, matrix metalloproteinase-2 and its tissue inhibitor in patients with pseudoexfoliation glaucoma/syndrome
Transforming growth factor β1, matrix metalloproteinase-2 and its tissue inhibitor in patients with pseudoexfoliation glaucoma/syndrome
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DOI：
10.2298/vsp1203231d
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发表时间：
2012-03-01
影响因子：
0.2
通讯作者：
Schlotzer-Schrehardt, Ursula
中科院分区：
文献类型：
--
作者：
Djordjevic-Jocic, Jasmina;Zlatanovic, Gordana;Schlotzer-Schrehardt, Ursula
背景/目标。转化生长因子-β 1（TGF-β 1）、氧化应激以及基质金属蛋白酶（MMPs）及其组织抑制剂（TIMP）之间的失衡可能在假性剥脱综合征/青光眼（PEX Sy/Gl）的发病机制中发挥重要作用。本研究的目的是测量检查组中房水中TGF-β 1、MMP-2、TIMP-2的浓度，并将生化结果与以下临床参数进行比较：房角色素沉着程度、假性剥脱的存在和眼内压（IOP）值。方法.在超声乳化白内障手术过程中收集了30例白内障患者、30例PEX Sy患者、36例PEX Gl患者和42例原发性开角型青光眼（POAG）患者的房水样本。TGF β 1、MMP-2、TIMP-2 Fluotokine Multi Analyze Profiling试剂盒和Luminex技术用于同时测量TGF β 1、MMP-2和TIMP-2。结果TGF-β 1、MMP-2、TIMP-2在所有组的人眼房水中均被检测到，其中PEX G1组的水平最高。PEX G1患者房水中TGF β 1、MMP-2、TIMP-2水平与眼压值之间存在显著相关性（p < 0.05）。在该组中，报道了一方面，房水中TGF β 1浓度与假性剥脱的存在之间的正相关性（p < 0.01），另一方面，TIMP-2水平与假性剥脱的存在之间的正相关性（p < 0.05）。在PEX G1组中，TGF-β 1和MMP-2与房角色素沉着程度的统计学显著正相关性得到证实（p < 0.05）。POAG组TIMP-2与色素沉着程度呈负相关（p < 0.05），与眼压呈负相关（p < 0.05）。结论TGF β 1和MMP-2影响假性剥脱性青光眼患者房角色素沉着程度和假性剥脱程度。
Background/Aim. Transforming growth factor-beta 1 (TGF-beta 1), oxidative stress and imbalance between matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) may play an important role in pathogenesis of pseudoexfoliation syndrome/glaucoma (PEX Sy/Gl). The aim of the study was to measure concentrations of TGF-beta 1, MMP-2, TIMP-2 in the aqueous humor in the examined group, as well as to compare the biochemical findings with the following clinical parameters: degree of chamber angle pigmantation, presence of pseudoexfoliation and the value of intraocular pressure (IOP). Methods. Aqueous samples from 30 patients with cataract, 30 patients with PEX Sy, 36 patients with PEX Gl, and 42 patients with primary open-angle glaucoma (POAG) were collected during phacoemulsification cataract surgery. TGF beta 1, MMP-2, TIMP-2 Fluotokine Multi Analyze Profiling kits and Luminex technology were used to simultaneously measure TGF beta 1, MMP-2 and TIMP-2. Results. TGF-beta 1, MMP-2, TIMP-2 were detected in human aqueous from all the groups with the highest level in the group with PEX Gl. Statistically, a significant correlation between the levels of TGF beta 1, MMP-2, TIMP-2 in the aqueous humor of the patients with PEX Gl and the IOP value was confirmed (p < 0.05). In this group, the positive correlations between the TGF beta 1 concentration in the aqueous humor and the presence of pseudoexfoliation (p < 0.01), on the one hand, and between the TIMP-2 level and the presence of pseudoexfoliation (p < 0.05), on the other, were reported. A statistically significant positive correlation of TGF-beta 1 and MMP-2, and the degree of chamber angle pigmentation in the PEX Gl group was confirmed (p < 0.05). In the POAG group, TIMP-2 values were in a negative correlation with the degree of pigmentation (p < 0.05), and the IOP value (p < 0.05). Conclusion. TGF beta 1 and MMP-2 affect the degree of chamber angle pigmentation and the degree of pseudoexfoliation in patients with pseudoexfoliative glaucoma.