Three-dimensional reconstruction in free-space whole-body fluorescence tomography of mice using optically reconstructed surface and atlas anatomy
Three-dimensional reconstruction in free-space whole-body fluorescence tomography of mice using optically reconstructed surface and atlas anatomy
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DOI：
10.1117/1.3258836
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发表时间：
2009-11-01
影响因子：
3.5
通讯作者：
Johnson, G. Allan
中科院分区：
文献类型：
--
作者：
Zhang, Xiaofeng;Badea, Cristian T.;Johnson, G. Allan
我们提出了一种三维图像重建方法的自由空间荧光断层扫描的小鼠使用混合解剖先验信息。具体来说，我们使用光学重建的表面的实验动物和数字鼠标图集近似的动物的解剖结构先验，以协助图像重建。实验是在一具裸小鼠尸体上进行的，该尸体的胸腔内植入了荧光内含物（直径2.4 mm的圆柱体）。层析荧光图像重建使用基于有限元方法的迭代算法。荧光重建和显微CT（计算机断层扫描）数据的配准后，获得了良好的定位精度（定位误差1.2 +/- 0.6毫米）。使用光学重建的表面，但没有图谱解剖，图像重建无法正确显示荧光夹杂物。该方法证明了在支持自由空间荧光断层扫描的解剖先验的效用。(C)2009年，由光学仪器工程师协会（Society of Photo-Optical Instrumentation Engineers）主办。[DOI电话：10.1117/1.3258836]
We present a 3-D image reconstruction method for free-space fluorescence tomography of mice using hybrid anatomical prior information. Specifically, we use an optically reconstructed surface of the experimental animal and a digital mouse atlas to approximate the anatomy of the animal as structural priors to assist image reconstruction. Experiments are carried out on a cadaver of a nude mouse with a fluorescent inclusion (2.4-mm-diam cylinder) implanted in the chest cavity. Tomographic fluorescence images are reconstructed using an iterative algorithm based on a finite element method. Coregistration of the fluorescence reconstruction and micro-CT (computed tomography) data acquired afterward show good localization accuracy (localization error 1.2 +/- 0.6 mm). Using the optically reconstructed surface, but without the atlas anatomy, image reconstruction fails to show the fluorescent inclusion correctly. The method demonstrates the utility of anatomical priors in support of free-space fluorescence tomography. (C) 2009 Society of Photo-Optical Instrumentation Engineers. [DOI: 10.1117/1.3258836]